PMID: 9539101Apr 16, 1998Paper

Enhanced detection of human telomerase activity

DNA and Cell Biology
J C NortonJ W Shay

Abstract

The detectability of telomerase activity in human cells almost always correlates with indefinite proliferation capability (immortalization). To make quantitative statements about telomerase activity levels, complete extraction of the telomerase activity is needed. A series of detergents was tested for this purpose, and a combination of NP-40 and sodium deoxycholate (NaDOC) was found to be the most efficient for extracting telomerase activity. Tumor-derived cell lines originally thought to contain differing amounts of telomerase on the basis of the original CHAPS bases extraction procedures have nearly equivalent amounts of activity when extracted with the NP-40/NaDOC lysis buffer. These results indicate that these lysis conditions can be used to extract telomerase activity more efficiently from tumor-derived cell lines.

Associated Clinical Trials

References

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Citations

Mar 10, 2001·The Journal of Pathology·J V BovéeP C Hogendoorn
Sep 21, 2000·Cancer Letters·L S Gollahon, S E Holt
Oct 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·C Orlando, S Gelmini
Apr 28, 1999·Molecular Medicine Today·K E McKenzieS Sukumar
Mar 18, 2000·Proceedings of the Society for Experimental Biology and Medicine·I Savre-TrainS E Holt
Feb 6, 2008·Zebrafish·Patricia A McChesneyShawn E Holt
Apr 10, 1999·Genes & Development·S E HoltM A White
Nov 14, 1998·Annals of Medicine·V UrquidiS Goodison
Jul 18, 2001·The Journal of Urology·C OrlandoM Pazzagli
Apr 4, 2003·Analytical Biochemistry·Sabita N SaldanhaTrygve O Tollefsbol
Oct 25, 2016·Journal of Applied Microbiology·K G KiranJ Angayarkanni
Jan 30, 2008·Current Protocols in Cell Biology·Brittney-Shea HerbertWoodring E Wright
Sep 10, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Lynne W ElmoreShawn E Holt
Apr 1, 2000·The Journal of Biological Chemistry·M M OuelletteW E Wright
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C PorembaW Boecker
May 16, 2008·Experimental Biology and Medicine·Lynne W ElmoreShawn E Holt
May 3, 2011·Cancer Research·Maria Antonietta CeroneAlan Ashworth

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