Enhanced detection of sulfo-peptides as onium salts in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Masaaki Ueki, Miyuki Yamaguchi

Abstract

A new two-component system, consisting of a matrix and an onium salt as comatrix, is described for detection of sulfo-peptides in the positive mode by matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Binary iodonium salts were superior to quaternary phosphonium salts in terms of suppression of desulfation and salt formation with the carboxyl group. Of the iodonium salts examined, bis(4-tert-butylphenyl)iodonium (BTI) hexafluorophosphate and bromide were most effective in giving intensive molecular ion signals in the form of [M(BTI)+BTI](+). The conditions optimized for O-sulfated tyrosine-containing peptides could be applicable for O-sulfated serine- and threonine-containing peptides. In the case of a phospho-peptide, a molecular ion appeared more intensively as a proton adduct than as a BTI adduct.

References

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Mar 16, 2001·Bioorganic & Medicinal Chemistry·M UekiH Ogawara
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Citations

Jan 31, 2007·Journal of Mass Spectrometry : JMS
Mar 18, 2011·Analytical Chemistry·Katherine S LovejoyRico E Del Sesto
Aug 27, 2015·European Journal of Mass Spectrometry·Kazuaki OharaKentaro Yamaguchi
Nov 1, 2006·Analytical Chemistry·Haichuan Liu, Kristina Håkansson

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