PMID: 11906736Mar 22, 2002Paper

Enhanced detection of Theiler's virus RNA copy equivalents in the mouse central nervous system by real-time RT-PCR

Journal of Virological Methods
Mark TrottierHoward L Lipton

Abstract

Infection of mice by low-neurovirulence Theiler's murine encephalomyelitis virus (TMEV), such as BeAn and DA viruses, provides a relevant experimental animal model for multiple sclerosis (MS). As a step toward determining the kinetics of a persistent central nervous system (CNS) infection that leads to chronic demyelination, we adapted a rapid, accurate and highly specific real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection and quantitation of BeAn virus RNA copy equivalents in mouse tissues. The assay enabled detection of as few as 20-30 copies of BeAn virus RNA per microg of total RNA from infected mouse tissues and results for spinal cord revealed the same high levels of BeAn RNA as detected by Northern hybridization during the first 4 months of the persistent infection, but also was able to detect virus RNA copies as late as 1 year post-infection. Real-time RT-PCR analysis of BeAn virus RNA copy equivalents in different parts of the CNS, analyses not possible by Northern hybridization, revealed the following cline of virus persistence: spinal cord>brainstem/cerebellum>cerebrospinal fluid (CSF)>cerebral hemispheres. Systemic organs, including heart, intestine and mesenteric lymph nodes of i...Continue Reading

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Citations

Nov 5, 2003·Analytical Biochemistry·Zhen Huang, Mohammed Alsaidi
Feb 5, 2003·Journal of Virological Methods·W James CookWilliam R Green
Jan 17, 2004·Clinical Microbiology Reviews·Emilia L OleszakChris D Platsoucas
May 6, 2011·Journal of Virology·Shannon HertzlerHoward L Lipton
Feb 1, 2013·Journal of Virology·Jingshan ZhangHarel Dahari
Aug 10, 2004·Chembiochem : a European Journal of Chemical Biology·Mohammed AlsaidiZhen Huang
Apr 4, 2003·Analytical Biochemistry·Zhen Huang, Jack W Szostak
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenna L Bowen, Julie K Olson
Aug 26, 2004·Multiple Sclerosis : Clinical and Laboratory Research·Paul W DentonRoger W Melvold

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