Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis.

Applied and Environmental Microbiology
Jong Hwan BaekSang Yup Lee

Abstract

A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.

References

Sep 1, 1991·Molecular Microbiology·D JeanteurF Pattus
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 12, 2001·Current Opinion in Biotechnology·K D Wittrup
Sep 5, 2002·Biotechnology and Bioengineering·Wilfred Chen, George Georgiou
Dec 14, 2002·Trends in Biotechnology·Sang Yup LeeZhaohui Xu
Oct 9, 2003·Applied and Environmental Microbiology·Mee-Jung HanSang Yup Lee
Jan 2, 2004·Journal of Bacteriology·Matthew P DeLisaGeorge Georgiou
Jul 28, 2005·Molecular Microbiology·Andrew J Darwin
Oct 6, 2006·FEMS Microbiology Letters·Jong Hwan Baek, Sang Yup Lee
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin Hwan ParkSang Yup Lee
Jun 28, 2008·Trends in Biotechnology·Jin Hwan ParkHyun Uk Kim

❮ Previous
Next ❯

Citations

Aug 12, 2014·Applied Microbiology and Biotechnology·Jan SchüürmannJoachim Jose
Jun 15, 2013·Journal of Bioscience and Bioengineering·Chia-Chi LinYung-Chuan Liu
Nov 29, 2013·Biotechnology and Bioengineering·Hyun Tae HwangArvind Varma
Sep 8, 2012·Applied Biochemistry and Biotechnology·Saisubramanian Nagarajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.