Enhanced DNA repair of bleomycin-induced 3'-phosphoglycolate termini at the transcription start sites of actively transcribed genes in human cells

Mutation Research
Vincent MurrayAnne M Galea

Abstract

The anti-tumour agent, bleomycin, cleaves DNA to give 3'-phosphoglycolate and 5'-phosphate termini. The removal of 3'-phosphoglycolate to give 3'-OH ends is a very important step in the DNA repair of these lesions. In this study, next-generation DNA sequencing was utilised to investigate the repair of these 3'-phosphoglycolate termini at the transcription start sites (TSSs) of genes in HeLa cells. The 143,600 identified human TSSs in HeLa cells comprised 82,596 non-transcribed genes and 61,004 transcribed genes; and the transcribed genes were divided into quintiles of 12,201 genes comprising the top 20%, 20-40%, 40-60%, 60-80%, 80-100% of expressed genes. Repair of bleomycin-induced 3'-phosphoglycolate termini was enhanced at actively transcribed genes. The top 20% and 20-40% quintiles had a very similar level of enhanced repair, the 40-60% quintile was intermediate, while the 60-80% and 80-100% quintiles were close to the low level of enhancement found in non-transcribed genes. There were also interesting differences regarding bleomycin repair on the sense and antisense strands of DNA at TSSs. The sense strand had highly enhanced repair between 0 and 250bp relative to the TSS, while for the antisense strand highly enhanced rep...Continue Reading

References

Apr 24, 1999·Biochimie·S Tornaletti, P C Hanawalt
Feb 19, 2002·Chemical Reviews·Richard M. Burger
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lawrence H Einhorn
Aug 30, 2003·Oncogene·Kristoffer Valerie, Lawrence F Povirk
Feb 3, 2005·Nature Reviews. Cancer·Jingyang Chen, JoAnne Stubbe
Jul 1, 2005·Nature·Tae Hoon KimBing Ren
Mar 4, 2006·Molecular Cancer Research : MCR·Jacqueline CloosBoudewijn J M Braakhuis
May 17, 2007·Expert Opinion on Therapeutic Targets·Nicola Curtin
Mar 11, 2008·Cell·Dustin E SchonesKeji Zhao
Jun 26, 2008·Annual Review of Genomics and Human Genetics·Elaine R Mardis
Nov 22, 2008·Nature Reviews. Molecular Cell Biology·Philip C Hanawalt, Graciela Spivak
Feb 11, 2009·Nature Reviews. Genetics·Cizhong Jiang, B Franklin Pugh
Mar 21, 2009·Current Opinion in Genetics & Development·Zhibin WangKeji Zhao
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jun 23, 2010·Chemical Biology & Drug Design·Anne M Galea, Vincent Murray
Feb 1, 2011·FEBS Letters·Natalia A LebedevaOlga I Lavrik
Apr 24, 2012·Biochimie·Natalia A LebedevaOlga I Lavrik
Feb 16, 2013·Nucleic Acids Research·Erbay YigitMatthew E Pipkin
May 21, 2013·DNA Repair·Teruaki Iyama, David M Wilson

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Citations

Dec 8, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Long H Chung, Vincent Murray
Feb 9, 2017·The International Journal of Biochemistry & Cell Biology·Jon K ChenVincent Murray
Mar 5, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jon K Chen, Vincent Murray
May 17, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shweta D GautamVincent Murray
May 8, 2018·International Journal of Molecular Sciences·Vincent MurrayLong H Chung
May 1, 2019·Molecular Biology Reports·Megan E HardieVincent Murray
May 18, 2016·Molecular Biology Reports·Vincent MurrayMark M Tanaka
Oct 26, 2016·Bioorganic & Medicinal Chemistry·Jon K ChenVincent Murray

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