Enhanced dynamic instability of microtubules in a ROS free inert environment

Biophysical Chemistry
Md Sirajul IslamAkira Kakugo

Abstract

Reactive oxygen species (ROS), one of the regulators in various biological processes, have recently been suspected to modulate microtubule (MT) dynamics in cells. However due to complicated cellular environment and unavailability of any in vitro investigation, no detail is understood yet. Here, by performing simple in vitro investigations, we have unveiled the effect of ROS on MT dynamics. By studying dynamic instability of MTs in a ROS free environment and comparing with that in the presence of ROS, we disclosed that MTs showed enhanced dynamics in the ROS free environment. All the parameters that define dynamic instability of MTs e.g., growth and shrinkage rates, rescue and catastrophe frequencies were significantly affected by the presence of ROS. This work clearly reveals the role of ROS in modulating MT dynamics in vitro, and would be a great help in understanding the role of ROS in regulation of MT dynamics in cells.

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Citations

Mar 23, 2017·Chemical Society Reviews·H Hess, Jennifer L Ross
Mar 4, 2020·Biophysics Reviews·Jakia Jannat KeyaAkira Kakugo
Sep 18, 2020·Science and Technology of Advanced Materials·Arif Md Rashedul KabirAkira Kakugo
Feb 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Lixin WangPeijie Gong
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Xin WenCheng-Guang Liang
Sep 12, 2019·Chemical Reviews·Gadiel Saper, Henry Hess

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