Enhanced effect of IFNgamma on the induced apoptosis of neuroblastoma cells by cytotoxic drugs

Pediatric Hematology and Oncology
Hai-xia TongJin-hua Zhang

Abstract

The objective of this study was to explore the antitumor effects of cytotoxic drugs combined with IFNgamma on neuroblastoma cell line SH-SY5Y cells. The expression of caspase 8 mRNA and protein was detected with RT-PCR and Western blot analysis. The effects of cytotoxic drugs and IFNgamma combined with cytotoxic drugs on the growth and apoptosis of SH-SY5Y cells were detected with the methods of MTT and flow cytometry. Caspase 8 activity was measured by colorimetric assay. Caspase 8 was undetectable in SH-SY5Y cells with an increased expression of caspase 8 after the treatment of IFNgamma. SH-SY5Y cells were sensitive to Adriamycin relatively but resistant to TNFalpha and TRAIL, while IFNgamma-pretreated SH-SY5Y cells were more sensitive to the cytotoxic drugs with an increase of caspase 8 activity. The authors conclude that IFNgamma can sensitize SH-SY5Y cells to Adriamycin-, TNFalpha-, and TRAIL-induced apoptosis and this may be realized by the upregulation of caspase 8.

References

Nov 5, 2002·Nature Reviews. Cancer·Mark J SmythHideo Yagita
Mar 19, 2004·Pathology Oncology Research : POR·Martin EbingerWolfram Scheurlen
Dec 7, 2005·International Journal of Cancer. Journal International Du Cancer·Keisuke TateishiMasao Omata
Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·M A MillerA D Sandler

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Citations

Mar 12, 2009·Journal of Cellular Biochemistry·Lars M Wagner, Mary K Danks
Sep 16, 2010·Current Opinion in Oncology·Fabrice AndreChristine Desmedt
Dec 4, 2009·Pediatric Hematology and Oncology·Haixia TongJinhua Zhang

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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