Enhanced effect of increased erythrocyte production rate on plasma erythropoietin levels of mice during subsequent exposure to hypobaria

Advances in Experimental Medicine and Biology
C E BozziniA C Barcelò

Abstract

The present study was undertaken to determine plasma EPO levels in response to acute exposure to hypobaria in transfused-polycythemic mice previously exposed to different forms of erythropoietic stimulation. The erythrocyte production rate (EPR) was stimulated by one of the following conditions: (1) discontinuous exposure to 456 mb during 4 weeks, (2) one weekly injection of phenylhydrazine (PHZ) during 4 weeks for induction of a compensated hemolytic state, and 3) three weekly injections of rHuEPO at a dose level of 1500 U/Kg body weight for 4 weeks. Treated and control mice received different volumes of packed red cells in order to obtain hematocrit values around 65% in all groups. Mice from each group were exposed to 456 mb during 14 h, 4 days after transfusion. Mice were bled immediately through cardiac puncture after removal from the hypobaric chamber. Plasma EPO titer was estimated by radioimmunoassay. Two observations were worth noting: (1) plasma iEPO level was very low in mice with transfusion polycythemia exposed to hypobaria, the value of 12 +/- 1.3 mU/ml being significantly different from that of 180 +/- 18 mU/ml found in normocythemic mice similarly exposed, and (2) plasma iEPO levels during EPRs were previously st...Continue Reading

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