Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Wenhui WangPing Liu

Abstract

A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover. Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment. A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved. Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed. X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment. The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disrupti...Continue Reading

References

Jul 2, 2003·Bioresource Technology·Tae Hyun KimY Y Lee
Nov 13, 2004·Biotechnology and Bioengineering·Yi-Heng Percival Zhang, Lee R Lynd
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael H StuderCharles E Wyman
Mar 27, 2013·Journal of Agricultural and Food Chemistry·Mahesh V BuleShulin Chen
Sep 21, 2013·Bioresource Technology·Anushadevi PanneerselvamThomas Ranney

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Citations

May 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shangyuan TangXiyu Cheng
Apr 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lingci LiXiyu Cheng
Aug 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xia ZhouPing Liu
Feb 5, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katja VasićMaja Leitgeb
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jihane ZeghlouliCédric Delattre

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Methods Mentioned

BETA
Infrared Spectroscopy
X-ray

Software Mentioned

PerkinElmer Spectrum
Origin

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