Enhanced enzymatic hydrolysis of sugarcane bagasse by N-methylmorpholine-N-oxide pretreatment

Bioresource Technology
Chia-Hung Kuo, Cheng-Kang Lee

Abstract

The cellulose dissolution solvent used in Lyocell process for cellulose fiber preparation, N-methylmorpholine-N-oxide (NMMO) monohydrate, was demonstrated to be an effective agent for sugarcane bagasse pretreatment. Bagasse of 20wt% was readily dissolved in NMMO monohydrate at 130 degrees C within 1h. After dissolution, bagasse could be regenerated by rapid precipitation with water as a porous and amorphous mixture of its original components. The regenerated bagasse exhibited a significant enhancement on enzymatic hydrolysis kinetic. Not only the reducing sugars releasing rate but also hydrolysis yield was enhanced at least twofold as compared with that of untreated bagasse. The cellulose fraction of regenerated bagasse was nearly hydrolyzed to glucose after 72h hydrolysis with Cellulase AP3. The recycled NMMO demonstrated the same performance as the fresh one on bagasse pretreatment for hydrolysis enhancement. The regenerated bagasse was directly used in simultaneous saccharification and fermentation (SSF) for ethanol production by Zymomonas mobilis. No negative effect on ethanol fermentation was observed and ethanol yield approximately 0.15 g ethanol/g baggasse was achieved.

References

Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Aug 23, 2005·Bioresource Technology·Tim Eggeman, Richard T Elander
Aug 19, 2006·Biotechnology and Bioengineering·Anantharam P DadiConstance A Schall
Feb 24, 2007·Biotechnology and Bioengineering·Yi-Heng Percival ZhangLee R Lynd
Nov 21, 2007·Biotechnology and Bioengineering·Yulin ZhaoYulin Deng

❮ Previous
Next ❯

Citations

Sep 15, 2010·Journal of Industrial Microbiology & Biotechnology·Marina O S DiasCarlos E V Rossell
Jul 25, 2014·Critical Reviews in Biotechnology·Naivy Y Nava-CruzCristóbal N Aguilar
Aug 10, 2010·Waste Management·Azam JeihanipourMohammad J Taherzadeh
Jun 4, 2010·Biotechnology and Bioengineering·Chandraraj KrishnanVenkatesh Balan
Jun 21, 2011·Enzyme Research·Gary BrodeurSubramanian Ramakrishnan
Aug 28, 2014·BioMed Research International·Marzieh ShafieiMohammad J Taherzadeh
Feb 23, 2013·Bioresource Technology·Tian-Qing LanXiaoguang Liu
Oct 21, 2016·Applied Microbiology and Biotechnology·Laura Capolupo, Vincenza Faraco
Feb 7, 2017·Bioresources and Bioprocessing·Adepu Kiran Kumar, Shaishav Sharma
Sep 15, 2015·ChemSusChem·Marcos Henrique Luciano SilveiraLuiz Pereira Ramos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.