Enhanced Escherichia coli adherence and invasion in Crohn's disease and colon cancer

Gastroenterology
Helen M MartinJonathan M Rhodes

Abstract

Altered mucosal glycosylation in inflammatory bowel disease and colon cancer could affect mucosal bacterial adherence. This study aimed to quantify and characterize mucosa-associated and intramucosal bacteria, particularly Escherichia coli, in these conditions. Mucosa-associated bacteria were isolated, after dithiothreitol mucolysis, from biopsy samples obtained at colonoscopy (Crohn's disease, n = 14 patients; ulcerative colitis, n = 21; noninflamed controls, n = 24) and at surgical resection (colon cancer, n = 21). Intramucosal bacteria were grown after gentamicin treatment followed by hypotonic lysis. Mucosa-associated and intramucosal bacteria were cultured more commonly in Crohn's disease (79%, P = 0.03; and 71%, P < 0.01, respectively), but not ulcerative colitis (38% and 48%), than in noninflamed controls (42% and 29%) and were commonly cultured from colon cancers (71% and 57%). Mucosa-associated E. coli, which accounted for 53% of isolates, were more common in Crohn's disease (6/14; 43%) than in noninflamed controls (4/24, 17%), as also were intramucosal E. coli: Crohn's disease, 29%; controls, 9%. E. coli expressed hemagglutinins in 39% of Crohn's cases and 38% of cancers but only 4% of controls, and this correlated (P...Continue Reading

References

Jul 9, 1988·BMJ : British Medical Journal·D A Burke, A T Axon
Dec 1, 1988·The Journal of Infectious Diseases·T E KingP A Campbell
Oct 1, 1996·Glycoconjugate Journal·Y S KimI Brockhausen
Jul 25, 1998·Gastroenterology·A SwidsinskiH Lochs
Dec 3, 1998·Gastroenterology·A Darfeuille-MichaudJ F Colombel
Oct 24, 2000·International Journal of Medical Microbiology : IJMM·P Klemm, M A Schembri
Aug 14, 2001·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S M Henry
Feb 14, 2002·Trends in Molecular Medicine·Jonathan M Rhodes, Barry J Campbell
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Senitiroh Hakomori
Aug 13, 2002·Gut·S M DawseyP J Limburg
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mary O'RiordanDaniel A Portnoy
Jan 16, 2003·The Journal of Biological Chemistry·Stephen E GirardinPhilippe J Sansonetti
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mathias ChamaillardGilles Thomas
Apr 3, 2003·Gastroenterology·Tadakazu HisamatsuDaniel K Podolsky

❮ Previous
Next ❯

Citations

Sep 18, 2010·Inflammatory Bowel Diseases·Janelle C Arthur, Christian Jobin
Oct 22, 2010·Mucosal Immunology·P D SmithS M Wahl
Nov 18, 2011·Mucosal Immunology·S KeelyP M Hansbro
May 10, 2012·Mucosal Immunology·A BatraB Siegmund
Feb 22, 2012·Nature Reviews. Gastroenterology & Hepatology·Indrani MukhopadhyaGeorgina L Hold
Sep 18, 2013·Nature Reviews. Gastroenterology & Hepatology·Sergey R KonstantinovMaikel P Peppelenbosch
Sep 4, 2012·Nature Reviews. Microbiology·George HajishengallisMichael A Curtis
May 23, 2007·The Journal of Experimental Medicine·Guangyu AnLijun Xia
Sep 10, 2009·The Journal of Experimental Medicine·Frédéric A CarvalhoArlette Darfeuille-Michaud
Oct 22, 2008·British Medical Bulletin·Paul KnightJonathan M Rhodes
Jan 7, 2011·The Journal of Infectious Diseases·Cynthia L Sears, Drew M Pardoll
Aug 21, 2012·Science·Janelle C ArthurChristian Jobin
Dec 12, 2007·Antimicrobial Agents and Chemotherapy·Sreedhar SubramanianBarry J Campbell
Jun 28, 2011·Applied and Environmental Microbiology·Margarita Martinez-MedinaChrista Ewers
Jul 25, 2006·Infection and Immunity·Kenneth W SimpsonYnte H Schukken
Oct 30, 2012·Journal of Bacteriology·Benoit Chassaing, Arlette Darfeuille-Michaud
Jul 28, 2012·Journal of Clinical Microbiology·Nadeem O KaakoushHazel M Mitchell
Oct 16, 2009·Journal of Clinical Microbiology·Margarita Martinez-MedinaJorge Blanco
Aug 16, 2006·Gut·Alexander SwidsinskiYvonne Dörffel
Apr 26, 2007·Gut·Sabine NudingEduard F Stange
Mar 24, 2009·Annual Review of Immunology·Yasmine Belkaid, Kristin Tarbell
Jun 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eunok ImSang Hoon Rhee
Mar 3, 2007·The Journal of Clinical Investigation·Warren StroberPeter Mannon
Feb 23, 2011·The Journal of Clinical Investigation·Benoit ChassaingArlette Darfeuille-Michaud
Sep 24, 2009·BMC Microbiology·Margarita Martinez-MedinaL Jesus Garcia-Gil
Mar 29, 2006·Journal of Translational Medicine·D L Mager
Mar 14, 2008·Infectious Agents and Cancer·Sara TravaglioneCarla Fiorentini
Mar 2, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Vladimir ZajacVladimir Krcmery
Aug 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Vladimir HolecVladimir Zajac

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.