Enhanced expression of beta 3-galactosyltransferase 5 activity is sufficient to induce in vivo synthesis of extended type 1 chains on lactosylceramides of selected human colonic carcinoma cell lines

Glycobiology
Chi-Hung LinKay-Hooi Khoo

Abstract

In general, an elevated expression of beta 3-galactosyltransferase (beta 3GalT) activity contributed by beta 3GalT5 correlates well with increased biosynthesis and expression of type 1 chain (Gal beta 1-3GlcNAc beta 1-) derivatives such as Lewis A and sialyl Lewis A, which are mostly recognized as terminal epitopes and not further extended. Most known beta 3-N-acetylglucosaminyltransferases show a higher activity toward extending type 2 chain (Gal beta 1-4GlcNAc beta 1-), and an over-expression of beta 3GalT5 could suppress the formation of the type 2 chain poly-N-acetyllactosaminoglycans. The potential of extending instead the predominant type 1 chain termini synthesized under such circumstances was, however, not investigated, partly due to technical difficulty in unambiguous identification of extended type 1 chains. Using an advanced mass spectrometry-based glycomic mapping and glycan sequencing approach, we show here that type 1 chains carried on the lacto-series glycosphingolipids of colonic carcinoma cells can be extended when the endogenous beta 3GalT activity relative to competing beta 4GalT activity, as defined against a common GlcNAc beta 1-3Gal beta 1-4Glc acceptor, is sufficiently high, as found in Colo205 and SW1116...Continue Reading

References

Jan 1, 1994·Methods in Enzymology·A DellH R Morris
Jul 12, 2003·The Journal of Biological Chemistry·Soichiro IsshikiHisashi Narimatsu
Oct 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Catherine A DunnDixie L Mager
Sep 19, 2006·Current Opinion in Structural Biology·Hisashi Narimatsu

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Citations

Sep 30, 2014·Journal of Pharmacological Sciences·Hiroshi Sakagami
Dec 20, 2015·Wiley Interdisciplinary Reviews. Cognitive Science·Victor R SchinaziDaniel-Robert Chebat

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