Enhanced expression of GM-CSF in differentiating eosinophils of atopic and atopic asthmatic subjects

American Journal of Respiratory Cell and Molecular Biology
G M GauvreauJ A Denburg

Abstract

Higher numbers of eosinophil/basophil colony-forming units (Eo/B CFU) are observed in blood of atopic individuals, and can be enhanced in atopic asthmatics by allergen-inhalation challenge. It is known that mature basophils and eosinophils synthesize cytokines relevant to allergic inflammation. To investigate the potential role of growth factors in allergic disease we examined the expression of the hemopoietic cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-5, in differentiating Eo/B colony cells from normal and atopic individuals, and from atopic asthmatics before and after allergen-inhalation challenge. Peripheral blood was collected from two normal and 12 atopic individuals, and also from 25 atopic asthmatics before and 24 h after allergen challenge. Nonadherent mononuclear cells were isolated and grown in semisolid growth medium. Eo/B colonies were selected and cytospins were prepared for immunocytochemical analysis of colony cells. Eo/B colonies, especially carbol chromotrope 2R+ cells, selected at Days 10, 14, and 18 from atopic donors contained messenger RNA for GM-CSF by combined in situ reverse transcription-polymerase chain reaction and cytochemistry, and demonstrated time-dep...Continue Reading

References

Dec 1, 1991·American Journal of Respiratory Cell and Molecular Biology·I OhnoM Jordana
Nov 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P G GibsonJ A Denburg
Oct 1, 1989·American Journal of Respiratory Cell and Molecular Biology·C VancheriM Jordana
Feb 1, 1989·The British Journal of Clinical Psychology·D M Houston
Sep 1, 1987·The American Review of Respiratory Disease·P M O'ByrneF E Hargreave
Sep 1, 1987·The American Review of Respiratory Disease·H OtsukaJ A Denburg
Aug 1, 1986·The Journal of Allergy and Clinical Immunology·H OtsukaJ A Denburg
Sep 1, 1985·The Journal of Allergy and Clinical Immunology·J A DenburgJ Bienenstock
May 1, 1995·International Archives of Allergy and Immunology·H U SimonK Blaser
Oct 1, 1995·The Journal of Allergy and Clinical Immunology·D L HamilosQ Hamid
Nov 1, 1994·American Journal of Respiratory and Critical Care Medicine·D J CousinsT H Lee
Feb 1, 1994·Current Opinion in Immunology·P F Weller
May 1, 1994·The Journal of Allergy and Clinical Immunology·A Girgis-GabardoJ Dolovich
Jan 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J N WinterM O'Gorman
Nov 1, 1996·American Journal of Respiratory Cell and Molecular Biology·R SehmiJ A Denburg
Sep 1, 1996·American Journal of Respiratory Cell and Molecular Biology·M D InmanP M O'Byrne

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Citations

Mar 15, 2002·Current Allergy and Asthma Reports·Darryl AdamkoRedwan Moqbel
Feb 11, 2004·Current Allergy and Asthma Reports·Darryl AdamkoRedwan Moqbel
Oct 18, 2003·The Journal of Allergy and Clinical Immunology·Margarita Martinez-Moczygemba, David P Huston
Apr 6, 2004·Immunology and Allergy Clinics of North America·Judah A Denburg, Paul K Keith
Dec 28, 2002·American Journal of Respiratory and Critical Care Medicine·Ling-Yi L ChangJames D Crapo
Jul 23, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G M GauvreauJ A Denburg
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A I FanatR Sehmi
May 1, 2005·Expert Review of Clinical Immunology·Gail M Gauvreau, Judah A Denburg
Apr 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J L BrownS J Wilson
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M W Al-RabiaG M Walsh
Dec 21, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R Moqbel, P Lacy
Oct 4, 2017·Frontiers in Medicine·Stephane EsnaultJames S Malter

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