Enhanced expression of heat shock protein 70 (hsp70) and heat shock factor 1 (HSF1) activation in rheumatoid arthritis synovial tissue. Differential regulation of hsp70 expression and hsf1 activation in synovial fibroblasts by proinflammatory cytokines, shear stress, and antiinflammatory drugs

The Journal of Clinical Investigation
G SchettG Steiner

Abstract

Heat shock proteins (hsp) have been repeatedly implicated to participate in the pathogenesis of rheumatoid arthritis (RA). Herein, we investigated the regulation of synovial hsp70 expression by analyzing the DNA-binding activity of heat shock transcription factor 1 (HSF1) as well as inducible hsp70 expression. Experiments were performed both on synovial tissue and on synovial fibroblast-like cells (SFC). Gel mobility shift analysis revealed increased HSF1 activation, and Western blotting and immunohistochemistry revealed increased hsp70 expression in RA synovial tissue, but not in synovial tissue derived from patients with osteoarthritis. Proinflammatory cytokines (TNF-alpha, IL-1alpha, IL-6), but not IFN-gamma or TGF-beta, induced activation of HSF1-DNA binding and hsp70 expression in cultivated SFC. Activation of HSF1 in SFC was accompanied by hyperphosphorylation and nuclear translocation of HSF1. Furthermore, shear stress also induced a complete heat shock response in cultivated synovial cells. In contrast, nonsteroidal antiinflammatory drugs triggered only an incomplete heat shock response, with HSF1 activation but not hsp70 induction, whereas steroids and immunosuppressive drugs did not affect the heat shock response at a...Continue Reading

References

Jul 1, 1992·International Immunology·E J HogervorstW van Eden
Mar 6, 1992·Science·D A JurivichR I Morimoto
May 1, 1992·British Journal of Rheumatology·F M BrennanM Feldmann
Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Feb 1, 1989·Annals of the Rheumatic Diseases·G TsoulfaP M Lydyard
Oct 13, 1995·The Journal of Biological Chemistry·D A JurivichJ F Welk
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·B S LeeR I Morimoto
May 5, 1995·The Journal of Biological Chemistry·C Giardina, J T Lis
Mar 1, 1995·The Journal of Clinical Investigation·E R de Graeff-MeederB J Zegers
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D KimG C Li
Aug 1, 1994·The Journal of Experimental Medicine·A E GoldfeldA Rao
Aug 12, 1994·Science·E Kopp, S Ghosh
Mar 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W J Welch
Dec 1, 1993·Annals of the Rheumatic Diseases·M N FarahatG S Panayi
Apr 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·M KöllerW König
Feb 1, 1993·Circulation Research·P F Davies, S C Tripathi
Feb 25, 1996·Experimental Cell Research·A Samali, T G Cotter
Dec 1, 1995·The Journal of Clinical Investigation·G SchettG Wick

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Citations

Jan 29, 2010·Zeitschrift für Rheumatologie·J DetertF Buttgereit
Jun 26, 2009·Modern Rheumatology·Mesut Ogrendik
Jul 23, 2008·Bulletin of Experimental Biology and Medicine·V A NazarovI Yu Malyshev
Sep 21, 2010·Inflammation·Xinjing LuoXianzhong Xiao
Jun 30, 2000·La Revue de médecine interne·B GranelP J Weiller
Oct 12, 2000·Molecular and Cellular Endocrinology·T IshikawaT Fujita
Mar 11, 2000·Immunology·S M TodrykR G Vile
Dec 14, 2011·The New England Journal of Medicine·Iain B McInnes, Georg Schett
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Martijn J C van HerwijnenFemke Broere
Sep 6, 2007·Molecular and Cellular Biology·Sandra KettnerTamás Schweighoffer
Jan 12, 2005·Arthritis Research & Therapy·Taro Kawai, Shizuo Akira
Oct 25, 2008·Arthritis Research & Therapy·Stefan K DrexlerBrian M Foxwell
Jan 1, 2010·Journal of Oral Microbiology·Juan Pablo Loyola-RodriguezGregory J Seymour
Aug 14, 2010·Mediators of Inflammation·A M Piccinini, K S Midwood
May 21, 2013·FEBS Letters·Gábor BaloghLászló Vígh
Nov 3, 2010·Expert Opinion on Biological Therapy·Felix I L Clanchy, Sandra M Sacre
Mar 22, 2016·Journal of Immunology Research·Jun Xu, Ulka Sachdev
Nov 26, 2009·Journal of Clinical Periodontology·Rita E Martinez-MartinezJuan Pablo Loyola-Rodríguez
Jul 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jin-Hong KimJang-Soo Chun
Dec 9, 2009·Seminars in Arthritis and Rheumatism·Min-Nung HuangKamal D Moudgil

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