Enhanced expression of mRNA for nuclear factor kappaB1 (p50) in CD34+ cells of the bone marrow in rheumatoid arthritis

Arthritis Research & Therapy
Shunsei HirohataNicholas Chiorazzi

Abstract

Bone marrow CD34+ cells from rheumatoid arthritis (RA) patients have abnormal capacities to respond to tumor necrosis factor (TNF)-alpha and to differentiate into fibroblast-like cells producing matrix metalloproteinase (MMP)-1. We explored the expression of mRNA for nuclear factor (NF)kappaB in RA bone marrow CD34+ cells to delineate the mechanism for their abnormal responses to TNF-alpha. CD34+ cells were purified from bone marrow samples obtained from 49 RA patients and 31 osteoarthritis (OA) patients during joint operations via aspiration from the iliac crest. The mRNAs for NFkappaB1 (p50), NFkappaB2 (p52) and RelA (p65) were examined by quantitative RT-PCR. The expression of NFkappaB1 mRNA in bone marrow CD34+ cells was significantly higher in RA than in OA, whereas there was no significant difference in the expression of mRNA for NFkappaB2 and RelA. The expression of NFkappaB1 mRNA was not correlated with serum C-reactive protein or with the treatment with methotrexate or oral steroid. Silencing of NFkappaB1 by small interfering RNA abrogated the capacity of RA bone marrow CD34+ cells to differentiate into fibroblast-like cells and to produce MMP-1 and vascular endothelial growth factor upon stimulation with stem cell fac...Continue Reading

References

Jan 1, 1988·Cancer Investigation·R BatailleT Commes
Oct 1, 1984·Hematological Oncology·J A Child, M R Kushwaha
Oct 31, 2000·Journal of the Neurological Sciences·M Kawai, S Hirohata
Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
May 16, 2002·Current Rheumatology Reports·Ulf Müller-LadnerSteffen Gay
Aug 11, 2004·Best Practice & Research. Clinical Haematology·S J Bingham, J J Moore

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Citations

Dec 16, 2006·Arthritis Research & Therapy·Fiona M McQueen, Benedikt Ostendorf
Jun 11, 2011·Arthritis Research & Therapy·Paraskevi E KostaMaria I Argyropoulou
May 1, 2009·Arthritis and Rheumatism·John A Hamilton, Paul P Tak
Nov 21, 2013·Modern Rheumatology·Shunsei HirohataHideki Yoshikawa

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Datasets Mentioned

BETA
GM-SCF
GM-CSF

Methods Mentioned

BETA
PCR
transfection
light microscopy
ELISA
flow cytometry
nuclear translocation

Software Mentioned

LightCycler
Oligo Primer Analysis

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