Enhanced expression of neutral endopeptidase (NEP) in airway epithelium in biopsies from steroid- versus nonsteroid-treated patients with atopic asthma

American Journal of Respiratory Cell and Molecular Biology
J K SontP J Sterk

Abstract

The expression of the endogenous neuropeptide-degrading enzyme, neutral endopeptidase (NEP; CALLA, CD10, E.C.3.4.24.11) on cultured human airway epithelial cells can be upregulated by corticosteroids. We examined whether NEP expression in the airway epithelium or lamina propria in bronchial biopsies is enhanced in atopic asthmatics on regular inhaled steroids as compared with those without steroid treatment. Forty nonsmoking adults (age 19 to 48 yr) with mild to moderate asthma (forced expiratory volume in 1 s > or = 50% pred., histamine PC20 range 0.02 to 7.6 mg/ml) with (n = 23) or without (n = 17) regular inhaled steroids treatment entered the study. Biopsies were taken at (sub)segmental level from the right lower lobe, the middle lobe, and the main carina. Immunohistochemical staining was performed on cryostat sections using the VIL-A1 monoclonal antibody against CD10 (NEP). Intra- and inter-observer repeatability of a semiquantitative scoring method was good as assessed by weighted kappa (kappa(w) ranging from 0.66 to 0.81). In the airway epithelium, NEP-positive sites were within the basal layer and, in contrast with studies applying other antibodies, also at apical sites and within the lamina propria. In both the epithel...Continue Reading

Citations

Mar 20, 2002·Current Allergy and Asthma Reports·G F Joos
Oct 10, 2009·Current Allergy and Asthma Reports·Kirsten C VerheinDavid B Jacoby
Sep 20, 2001·International Immunopharmacology·A D Kraneveld, F P Nijkamp
Apr 27, 2000·Allergy·G F JoosR A Pauwels
Oct 29, 1998·Mediators of Inflammation·V H van der Velden
Apr 1, 1998·American Journal of Respiratory and Critical Care Medicine·P J BarnesW W Busse
Jan 18, 2003·American Journal of Respiratory and Critical Care Medicine·Laurent BenayounMarina Pretolani
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·A DruilheM Pretolani
Feb 15, 2005·Pulmonary Pharmacology & Therapeutics·Piera BoschettoCristina E Mapp
Apr 15, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·V H van der Velden, A R Hulsmann
Mar 22, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W I De BoerP J Sterk
May 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·V H Van Der VeldenM A Versnel
Jun 26, 2001·Respirology : Official Journal of the Asian Pacific Society of Respirology·A J RiceR Scicchitano
Jun 10, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K GrünbergJ H Van Krieken
Sep 25, 1999·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·E R DixonD B Lew
Mar 6, 2021·Neuroscience Letters·Alexandra B PincusDavid B Jacoby
May 30, 2020·Biochemical Pharmacology·Manar Mohammed El Tabaa, Maram Mohammed El Tabaa
Oct 14, 2000·The Journal of Allergy and Clinical Immunology·H W ChuR J Martin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

Related Papers

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
G F JoosR Pauwels
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
N CrimiA Mistretta
American Journal of Respiratory and Critical Care Medicine
C M LillyJ M Drazen
© 2021 Meta ULC. All rights reserved