Enhanced expression of phospholipase C 1 (ZmPLC1) improves drought tolerance in transgenic maize.

Planta
Chun-Rong WangJu-Ren Zhang

Abstract

Phosphatidylinositol-specific phospholipase C (PI-PLC) plays an important role in a variety of physiological processes in plants, including drought tolerance. It has been reported that the ZmPLC1 gene cloned from maize (Zea mays L.) encoded a PI-PLC and up-regulated the expression in maize roots under dehydration conditions (Zhai SM, Sui ZH, Yang AF, Zhang JR in Biotechnol Lett 27:799-804, 2005). In this paper, transgenic maize expressing ZmPLC1 transgenes in sense or antisense orientation were generated by Agrobacterium-mediated transformation and confirmed by Southern blot analysis. High-level expression of the transgene was confirmed by real-time RT-PCR and PI-PLC activity assay. The tolerance to drought stress (DS) of the homogenous transgenic maize plants was investigated at two developmental stages. The results demonstrated that, under DS conditions, the sense transgenic plants had higher relative water content, better osmotic adjustment, increased photosynthesis rates, lower percentage of ion leakage and less lipid membrane peroxidation, higher grain yield than the WT; whereas those expressing the antisense transgene exhibited inferior characters compared with the WT. It was concluded that enhanced expression of sense Zm...Continue Reading

References

Nov 18, 1976·Biochimica Et Biophysica Acta·J D Owen
Oct 25, 1988·Nucleic Acids Research·R Höfgen, L Willmitzer
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T HirayamaK Shinozaki
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·I StaxenM R McAinsh
Mar 9, 1999·Plant Physiology·S M Assmann, K i Shimazaki
Jun 6, 2000·Trends in Plant Science·J M StevensonW F Boss
Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·H J MeijerT Munnik
May 4, 2001·Trends in Plant Science·T Munnik
May 23, 2001·Current Biology : CB·C W Taylor, P Thorn
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 29, 2003·The Plant Journal : for Cell and Molecular Biology·Lee HuntJulie E Gray
Jun 14, 2003·Plant Physiology·Ryan N BurnetteGlenda E Gillaspy
Aug 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Fouad Lemtiri-ChliehCharles A Brearley
Jul 1, 1954·The Biochemical Journal·E W YEMM, A J WILLIS
Sep 25, 2003·Annual Review of Plant Biology·Harold J G Meijer, Teun Munnik
Dec 16, 2003·Journal of Experimental Botany·Lewis N MillsAlistair M Hetherington
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Peter C ScacheriFrancis S Collins
Feb 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Huixia ShouKan Wang
Sep 28, 2004·The Plant Journal : for Cell and Molecular Biology·Alexander ReppElmar Hartmann
Jul 19, 2005·Trends in Plant Science·Christa Testerink, Teun Munnik
Oct 29, 1982·Science·J S Boyer
Mar 1, 2003·Functional Plant Biology : FPB·Manuela M ChavesJoão S Pereira

❮ Previous
Next ❯

Citations

Apr 23, 2011·Journal of Experimental Botany·Marta S LopesChristine H Foyer
Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang
Oct 20, 2011·The Plant Journal : for Cell and Molecular Biology·Shu-Zhi ZhengDa-Ye Sun
Apr 30, 2010·Plant, Cell & Environment·Teun Munnik, Joop E M Vermeer
Dec 9, 2009·Plant, Cell & Environment·Yueyun HongXuemin Wang
May 3, 2015·Cell Calcium·Amarjeet SinghGirdhar K Pandey
Jun 26, 2009·The Biochemical Journal·Hong-Wei XueYu Mei
Aug 18, 2012·Plant Signaling & Behavior·J Armando Muñoz-SánchezS M Teresa Hérnandez-Sotomayor
May 23, 2016·BMC Plant Biology·Alessandra FracassoStefano Amaducci
Oct 21, 2017·International Journal of Molecular Sciences·Xiaoli WangShaojun Dai
Sep 4, 2018·Plant, Cell & Environment·Xianjun DengXuemin Wang
Apr 13, 2019·Journal of Experimental Botany·Liujie WuHiroyuki Koyama
Mar 21, 2019·International Journal of Molecular Sciences·Quan WangXinguo Li
Dec 14, 2018·Frontiers in Plant Science·Ringo van WijkTeun Munnik
May 16, 2020·Journal of Experimental Botany·Akinori KibaYasufumi Hikichi
Jan 13, 2021·Physiologia Plantarum·Marylou MantovaJosé M Torres-Ruiz
Jan 30, 2021·Frontiers in Genetics·Jiantang ZhuHui Li
Jun 3, 2021·Plants·Víctor M González-MendozaS M Teresa Hernández-Sotomayor
Aug 23, 2021·Journal, Genetic Engineering & Biotechnology·Madhvi KanchanJaspreet K Sembi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.