Enhanced expression of suppresser of cytokine signaling 3 inhibits the IL-6-induced epithelial-to-mesenchymal transition and cholangiocarcinoma cell metastasis

Medical Oncology
Qing-Xin ZhouYun-Fu Cui

Abstract

It was recently demonstrated that interleukin-6 (IL-6) induces the epithelial-to-mesenchymal transition (EMT) in cholangiocarcinoma (CCA), but the underlying molecular mechanism remains to be explored. In this study, we studied the role of suppresser of cytokine signaling 3 (SOCS3), a negative feedback regulator of IL-6/STAT3, in the IL-6-induced EMT in CCA. Treatment with IL-6 induced the EMT by decreasing the E-cadherin expression and increasing the expression of N-cadherin and vimentin. Using wound healing and invasion assays, we found that IL-6 promoted cell motility. Further, a stably transfected cell line overexpressing SOCS3 was constructed. Enhanced SOCS3 expression decreased IL-6-induced cell invasion and EMT in parallel with downregulating the IL-6/STAT3 pathway. In contrast, SOCS3 silencing using siRNA exhibited no effect on the cell invasive ability and EMT. Finally, an in vivo study indicated that the enhancement of SOCS3 expression decreased metastasis compared with the control, and this effect was achieved by the repression of p-STAT3, N-cadherin and vimentin, and the induction of E-cadherin assessed by Western blot analysis. Our results suggest that enhanced expression of SOCS3 can antagonize IL-6-induced EMT an...Continue Reading

References

Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gregory J Gores
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Biao HeDavid M Jablons
Jun 12, 2004·Seminars in Liver Disease·Oliver NehlsBodo Klump
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hajime IsomotoGregory J Gores
Apr 26, 2007·Annals of Surgery·Michelle L DeOliveiraRichard D Schulick
Aug 19, 2008·Seminars in Cell & Developmental Biology·Ben A CrokerSandra E Nicholson
Feb 3, 2009·Cancer Cell·Jacqueline Bromberg, Timothy C Wang
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jan 6, 2010·EMBO Molecular Medicine·Jose Miguel López-Novoa, M Angela Nieto
Feb 26, 2011·Annals of the Rheumatic Diseases·Sergei I Grivennikov, Michael Karin
Jan 19, 2012·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Stephanie Hiu Yan Lau, Wan Yee Lau
Jan 10, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Daisaku YamadaMasaki Mori
Sep 27, 2013·JAK-STAT·Kyoko Inagaki-OharaAkihiko Yoshimura
Oct 22, 2013·Gastroenterology·Sumera Rizvi, Gregory J Gores
Oct 5, 2014·The Lancet Oncology·Connie I DiakosStephen J Clarke

❮ Previous
Next ❯

Citations

Dec 26, 2015·Journal of Clinical Medicine·Simone BrivioMario Strazzabosco
May 20, 2016·Mediators of Inflammation·Samuela CataldiElisabetta Albi
Jul 30, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Claudio PintoMarco Marzioni
Oct 21, 2015·PloS One·Yimin WangYunfu Cui
Oct 27, 2015·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Zhongxue YeXu Li
Jan 31, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Yoshitaka KimuraHajime Kono
Jul 30, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hongyan CuiJingyun Wang
Oct 27, 2017·Gene Expression·Simone BrivioMario Strazzabosco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.