Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

Metabolic Engineering Communications
Harry R BellerJay D Keasling

Abstract

Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H2S, while fixing CO2are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering of the chemolithoautotrophic bacteriumThiobacillus denitrificansto produce up to 52-fold more fatty acids than the wild-type strain when grown with thiosulfate and CO2. A modified thioesterase gene fromE. coli('tesA) was integrated into theT. denitrificanschromosome under the control of Pkanor one of two nativeT. denitrificanspromoters. The relative strength of the two native promoters as assessed by fatty acid production in engineered strains was very similar to that assessed by expression of the cognate genes in the wild-type strain. This proof-of-principle study suggests that engineering sulfide-oxidizing chemolithoautotrophic bacteria to overproduce fatty acid-derived products merits consideration as a technology that could simultaneously produce renewable fuels/chemicals as well as cost-effectively remediate sulfide-contaminated wastewater.

References

Mar 3, 1995·The Journal of Biological Chemistry·H Cho, J E Cronan
Jun 30, 2006·Biotechnology and Bioengineering·Ricardo Beristain CardosoJim A Field
Mar 6, 2007·Applied and Environmental Microbiology·Tracy E LetainHarry R Beller
Apr 1, 1987·Biotechnology and Bioengineering·K L Sublette, N D Sylvester
Mar 15, 1991·Biotechnology and Bioengineering·C OngcharitY T Shah
Dec 30, 2009·Applied and Environmental Microbiology·Harry R BellerJay D Keasling
Sep 3, 2010·Applied Microbiology and Biotechnology·Ming-Fei ShaoHerbert Han-Ping Fang
May 21, 2013·Applied and Environmental Microbiology·Jana MüllerHarry R Beller
Jul 29, 2015·Natural Product Reports·Harry R BellerLeonard Katz

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical treatment
PCR
PCRs

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Environmental Science & Technology
S Miller
© 2022 Meta ULC. All rights reserved