Enhanced fluidity liquid chromatography: A guide to scaling up from analytical to preparative separations

Journal of Chromatography. a
Raffeal BennettErik L Regalado

Abstract

The evolution of supercritical fluid chromatography (SFC) instrumentation, improved detection capability, and expanded modifier range has led to extending the reach of SFC to the analysis of a broader spectrum of analytes beyond enantioselective separations. However, preparative SFC has yet to see the same technological revitalization, especially in regards to the purification of highly polar analytes. Enhanced fluidity liquid chromatography (EFLC) has been demonstrated as one of the ways to extend the applicable range of SFC instrumentation to highly polar analytes such as proteins, carbohydrates, and nucleotides. Despite recent applications of EFLC for challenging mixtures of hydrophilic metabolites and analogs, its viability in preparative purification, which is of great importance to the pharmaceutical industry, remains unknown. Herein, multiple chromatographic parameters that are critical to achieve feasible EFLC purification methods were investigated, including system pressure as a function of modifier composition (for several MeOH:H2O ratios), effect of diluent injection conditions on peak shape, and optimization of mass load with diluent composition. The usage of 50% acetonitrile or methanol diluents provided the most v...Continue Reading

Citations

May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert K HofstetterAndreas Link
Sep 18, 2020·Advances in Colloid and Interface Science·Shixiang LiuHailin Cong
Nov 12, 2020·Journal of Pharmaceutical and Biomedical Analysis·Jérémy MolineauCaroline West
May 1, 2020·Journal of Pharmaceutical and Biomedical Analysis·Manuel Ventura
Mar 25, 2021·Analytical Chemistry·Charles Phillip ShelorPurnendu K Dasgupta
Oct 23, 2019·Analytical Chemistry·Alexander S KaplitzJames P Grinias
Oct 29, 2019·Analytical Chemistry·Daipayan RoyDaniel W Armstrong

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