PMID: 2502576Jul 6, 1989Paper

Enhanced fluorescence in indirect immunophenotyping by the use of fluorescent liposomes

Journal of Immunological Methods
A GrayE R Huehns

Abstract

Small unilamellar liposomes were optimised for cell phenotyping by indirect immunofluorescence. This involved selection and covalent attachment to the liposome of a polyspecific ligand for cell-bound antibody. For this purpose sheep anti-mouse antibody was preferred to protein A because of its ability to attach to cell-bound IgG1 as well as IgG2 at physiological pH. The maximally fluorescent concentration of encapsulated carboxyfluorescein was determined to be 20 mM and liposomes thus comprised gave up to a nine-fold increase in mean cell fluorescence when compared with sheep anti-mouse antibody conjugated to fluorescein isothiocyanate. There was no parallel increase in background fluorescence. Liposomes retained their targeting and fluorescence properties after 3 months storage. They could be sterilised and were as versatile in use as FITC-antibody conjugates.

References

Jun 20, 1972·Biochimica Et Biophysica Acta·D PapahadjopoulosS Oki
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·J BarbetL D Leserman

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Citations

Jan 1, 1989·Current Opinion in Immunology·P M Lydyard
Aug 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·A ScheffoldA Radbruch
Dec 1, 1991·Blood Reviews·A Gray, J Morgan

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