Enhanced flux of substrates into polyamine biosynthesis but not ethylene in tomato fruit engineered with yeast S-adenosylmethionine decarboxylase gene

Amino Acids
Yi LasanajakA K Mattoo

Abstract

S-adenosylmethionine (SAM), a major substrate in 1-C metabolism is a common precursor in the biosynthetic pathways of polyamines and ethylene, two important plant growth regulators, which exhibit opposing developmental effects, especially during fruit ripening. However, the flux of various substrates including SAM into the two competing pathways in plants has not yet been characterized. We used radiolabeled (14)C-Arg, (14)C-Orn, L-[U-(14)C]Met, (14)C-SAM and (14)C-Put to quantify flux through these pathways in tomato fruit and evaluate the effects of perturbing these pathways via transgenic expression of a yeast SAM decarboxylase (ySAMDC) gene using the fruit ripening-specific promoter E8. We show that polyamines in tomato fruit are synthesized both from Arg and Orn; however, the relative contribution of Orn pathway declines in the later stages of ripening. Expression of ySAMDC reversed the ripening associated decline in spermidine (Spd) and spermine (Spm) levels observed in the azygous control fruit. About 2- to 3-fold higher levels of labeled-Spd in transgenic fruit (556HO and 579HO lines) expressing ySAMDC confirmed the enzymatic function of the introduced gene. The incorporation of L-[U-(14)C]Met into Spd, Spm, ethylene and...Continue Reading

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Citations

Jan 17, 2016·Plant Physiology and Biochemistry : PPB·Georgios TsaniklidisCostas Delis
Mar 1, 2018·Plant & Cell Physiology·Leandro Francisco de OliveiraEny Iochevet Segal Floh
May 28, 2019·Plant Biotechnology Journal·Sonia OsorioJocelyn K C Rose
Jan 1, 2017·Amino Acids·E Sobieszczuk-Nowicka
Aug 28, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Tadeu Dos Reis OliveiraClaudete Santa-Catarina
Nov 17, 2020·Frontiers in Plant Science·Seanna Hewitt, Amit Dhingra
Mar 6, 2021·Frontiers in Plant Science·Fan GaoYuanyue Shen

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