Enhanced FTIR bench-top imaging of single biological cells

The Analyst
C HughesPeter Gardner

Abstract

A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.

References

May 23, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Abigail V RutterJosep Sulé-Suso

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Citations

Mar 22, 2016·Chemical Society Reviews·Michael Pilling, Peter Gardner
Apr 15, 2015·Trends in Biotechnology·Caryn HughesMatthew J Baker
Apr 22, 2019·The Journal of Chemical Physics·Alex J SchofieldCarol J Hirschmugl
Jul 23, 2018·Analytical and Bioanalytical Chemistry·K L Andrew ChanGianfelice Cinque
May 1, 2020·Analytical and Bioanalytical Chemistry·Sabrina DiehnJanina Kneipp
Jul 21, 2015·Optics Express·Arnaldo L SerranoMartin T Zanni
Aug 31, 2017·Analytical and Bioanalytical Chemistry·James A Kimber, Sergei G Kazarian
Sep 29, 2018·Applied Spectroscopy·Susanne PahlowRohit Bhargava
Dec 28, 2017·Analytical Chemistry·Tomasz P Wrobel, Rohit Bhargava

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