PMID: 15381846Sep 24, 2004Paper

Enhanced functional and structural domain assignments using remote similarity detection procedures for proteins encoded in the genome of Mycobacterium tuberculosis H37Rv

Journal of Biosciences
Seema NambooriShashi Bhushan Pandit

Abstract

The sequencing of the Mycobacterium tuberculosis (MTB) H37Rv genome has facilitated deeper insights into the biology of MTB, yet the functions of many MTB proteins are unknown. We have used sensitive profile-based search procedures to assign functional and structural domains to infer functions of gene products encoded in MTB. These domain assignments have been made using a compendium of sequence and structural domain families. Functions are predicted for 78 % of the encoded gene products. For 69 % of these, functions can be inferred by domain assignments. The functions for the rest are deduced from their homology to proteins of known function. Superfamily relationships between families of unknown and known structures have increased structural information by approximately 11%. Remote similarity detection methods have enabled domain assignments for 1325 'hypothetical proteins'. The most populated families in MTB are involved in lipid metabolism, entry and survival of the bacillus in host. Interestingly, for 353 proteins, which we refer to as MTB-specific, no homologues have been identified. Numerous, previously unannotated, hypothetical proteins have been assigned domains and some of these could perhaps be the possible chemothera...Continue Reading

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Citations

Feb 17, 2006·PLoS Genetics·Julie C Dunning HotoppHervé Tettelin
Apr 24, 2021·Scientific Reports·Chintalapati JanakiNarayanaswamy Srinivasan
Feb 5, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nicola MulderVenu Vuppu

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