Enhanced gap junction expression in myoblast-containing engineered tissue.

Biochemical and Biophysical Research Communications
Sureshkumar Perumal SrinivasanYeong-Hoon Choi

Abstract

Transplantation of skeletal myoblasts (SMs) has been investigated as a potential cardiac cell therapy approach. SM are available autologously, predetermined for muscular differentiation and resistant to ischemia. Major hurdles for their clinical application are limitations in purity and yield during cell isolation as well as the absence of gap junction expression after differentiation into myotubes. Furthermore, transplanted SMs do not functionally or electrically integrate with the host myocardium. Here, we describe an efficient method for isolating homogeneous SM populations from neonatal mice and demonstrate persistent gap junction expression in an engineered tissue. This method resulted in a yield of 1.4 × 10(8) high-purity SMs (>99% desmin positive) after 10 days in culture from 162.12 ± 11.85 mg muscle tissue. Serum starvation conditions efficiently induced differentiation into spontaneously contracting myotubes that coincided with loss of gap junction expression. For mechanical conditioning, cells were integrated into engineered tissue constructs. SMs within tissue constructs exhibited long term survival, ordered alignment, and a preserved ability to differentiate into contractile myotubes. When the tissue constructs wer...Continue Reading

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Citations

Apr 16, 2014·International Journal of Tissue Engineering·Jason TchaoKimimasa Tobita
May 24, 2015·Cardiac Electrophysiology Clinics·Shone O AlmeidaReza Ardehali
Dec 3, 2014·The Journal of Thoracic and Cardiovascular Surgery·Philipp TreskesYeong-Hoon Choi
Dec 29, 2013·Stem Cells Translational Medicine·Narmeen HassanKimimasa Tobita
Mar 1, 2019·Bioelectricity·Colin Fennelly, Shay Soker

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