Enhanced glycosyltransferase activity during thermotolerance development in mammalian cells

Journal of Cellular Physiology
K J HenleA Stone

Abstract

The cellular heat shock response leads to the enhanced synthesis of a family of heat shock proteins and the development of thermotolerance. In CHO cells, however, heat shock also leads to enhanced synthesis of a 50 kD glycoprotein and elevated activity of N-acetylgalactosaminyltransferase (GalNAcT). In this study we showed increased GalNAcT activity during thermotolerance expression in all of five mammalian cell lines included in the study. However, there was no simple correlation between cellular heat sensitivity of unheated control cells and basal levels of GalNAcT activity, measured toward the same exogenous acceptor apomucin. Although GalNAcT was elevated in thermotolerant cells, GalNAcT activity itself did not exhibit thermotolerance in terms of reduced sensitivity to heat inactivation. The increase in GalNAcT activity after heating was similar in exponentially growing and plateau-phase cultures and was inhibited neither by cycloheximide nor actinomycin D. However, the inhibitors by themselves also increased GalNAcT activity in unheated control cells. Chemical inducers of thermotolerance (arsenite and diamide) increased GalNAcT activity, but the increase was modest when compared to that following hyperthermia. In addition ...Continue Reading

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Citations

May 1, 1991·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·K J HenleC K Lumpkin
Nov 1, 1990·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·K J HenleG T Nolen
Jun 1, 1991·International Journal of Radiation Oncology, Biology, Physics·T P MonsonA Mansouri
Nov 1, 1991·Journal of Cellular Physiology·Y J LeeP M Corry
Oct 1, 1991·Experimental Cell Research·K J Henle, W A Nagle

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