Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms

International Journal of Nanomedicine
Haoxiang ChenZhikai Tan

Abstract

Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells. Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells. The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG). The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration.

Citations

May 22, 2020·Journal of Polymers and the Environment·Alina GhilanVlad Mihai Chiriac
Oct 8, 2020·Advanced Healthcare Materials·Amelie ErbenMichael Heymann
Oct 4, 2020·Bioengineering·Gregory ReidMichela Pozzobon
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernando Alvarado-HidalgoRicardo Starbird-Perez
Feb 26, 2021·Journal of Tissue Engineering·Juan M Fernández-CostaJavier Ramón-Azcón
Jun 20, 2021·Current Opinion in Pharmacology·Kerrie DowningMarco Brotto
Dec 16, 2020·ACS Biomaterials Science & Engineering·Matthew H CleggElizabeth Vargis
Nov 18, 2021·Advanced Science·Claire BomkampElizabeth A Specht

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Methods Mentioned

BETA
irradiating
fluorescence-activated cell sorting
PCR
scanning electron microscopy
flow cytometry

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