Enhanced Homing Ability and Retention of Bone Marrow Stromal Cells to Diabetic Nephropathy by Microbubble-Mediated Diagnostic Ultrasound Irradiation

Ultrasound in Medicine & Biology
Gong WangYunhua Gao

Abstract

Bone marrow stromal cell (BMSC) transplantation can successfully treat diabetic nephropathy (DN), but the lack of a specific homing place for intravenously injected cells limits the effective implementation of stem cell therapies. The migration and survival of transplanted BMSCs are determined by inflammatory reactions in the local kidney micro-environment. We tested the hypothesis that microbubble-mediated diagnostic ultrasound irradiation could provide a suitable micro-environment for BMSC delivery and retention in DN therapy. In this study, red fluorescent protein-labeled BMSCs were administered combined with microbubbles to streptozotocin-induced DN rats 4 wk after diabetes onset. We observed enhanced BMSC homing and retention in microbubble-mediated diagnostic ultrasound-irradiated kidneys compared with the contralateral kidneys on days 1 and 3 post-treatment. The results from immunohistochemical analysis, Western blot and enzyme-linked immunosorbent assay indicated that the local and transient expression of various chemo-attractants (i.e., cytokines, integrins and trophic factors) found to promote BMSC homing was much higher than observed in non-treated kidneys. The local capillary endothelium rupture observed by transmis...Continue Reading

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Citations

Apr 17, 2018·Journal of Drug Targeting·Chaopin YangZhiyi Chen
Sep 20, 2017·Journal of the American Society of Nephrology : JASN·Travis D HullKenneth Hoyt
Mar 12, 2020·Stem Cells Translational Medicine·Daniel D LiuAvnesh S Thakor
May 24, 2019·IScience·Mujib UllahAvnesh S Thakor
Feb 13, 2021·Expert Opinion on Biological Therapy·Michail E KlontzasApostolos H Karantanas
Aug 7, 2021·Emerging Topics in Life Sciences·David Kuntin, Paul Genever

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