Enhanced humoral and cellular immune responses after sublingual immunization against human papillomavirus 16 L1 protein with adjuvants

Vaccine
Hee-Jeong ChoYu-Kyoung Oh

Abstract

Needle-free nonparenteral vaccines offer a number of practical advantages, especially in developing countries. To address the effects of vaccine administration route, we tested mucosal and systemic immune responses against human papillomavirus 16 L1(HPV16L1) protein using intranasal, intravaginal, transdermal, sublingual (SL) and intramuscular routes. The SL route provided the most effective mucosal secretory IgA (sIgA) and serum IgG responses. After a 150 microg antigen dose via the SL route, saliva sIgA levels were 7.2- and 5.8-fold higher than those achieved via intravaginal and transdermal routes, respectively. Notably, SL administration even produced 4.6-fold higher levels of vaginal sIgA levels than did intravaginal delivery of 150 microg antigen. To enhance the immunogenicity of SL vaccines, we tested the adjuvanticity of nine molecules: three toll-like receptor agonists, three nucleotide-binding oligomerization-domain agonists, vitamin D3, poly-gamma-glutamic acid and cholera toxin subunit B (CTB). Among the molecules tested, CTB provided the most enhanced mucosal sIgA and systemic IgG induction. SL-applied CTB enhanced the production of interleukin-4 and interferon-gamma from stimulated CD4+ T cells. Moreover, interfer...Continue Reading

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Citations

Nov 16, 2012·Expert Review of Vaccines·Ayman GebrilValerie A Ferro
Aug 28, 2015·Expert Review of Vaccines·Lu ZhangShixia Wang
Apr 20, 2012·Expert Opinion on Drug Delivery·Sevda ŞenelIndiran Pather
Jun 10, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Heleen KraanJean-Pierre Amorij
Feb 11, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Akhilesh Kumar ShakyaHarvinder Singh Gill
Jan 22, 2011·Expert Opinion on Therapeutic Patents·Hee-Jeong ChoYoung Bong Kim
Apr 13, 2012·Human Vaccines & Immunotherapeutics·Gabriel Pedersen, Rebecca Cox
Dec 12, 2018·Advanced Healthcare Materials·Rachel L Creighton, Kim A Woodrow
Jul 26, 2012·Nature Reviews. Immunology·Nils Lycke
Feb 27, 2021·Advanced Healthcare Materials·Sean H KellyJoel H Collier

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