Enhanced Humoral Responses Induced by Targeting of Antigen to Murine Dendritic Cells

Scandinavian Journal of Immunology
Lotte Hatting PugholmRalf Agger

Abstract

Dendritic cells (DCs) are superior in their ability to induce and control adaptive immune responses. These qualities have motivated the hypothesis that targeted delivery of antigen to DCs in vivo may be an effective way of enhancing immunization. Recent results show that antigen targeted to certain DC surface molecules may indeed induce robust immune responses. Targeting of antigen to DCs can be accomplished by the means of monoclonal antibodies. This study compared the humoral responses induced in mice by in vivo targeting of DCs using monoclonal antibodies specific for CD11c, CD36, CD205, Clec6A, Clec7A, Clec9A, Siglec-H and PDC-TREM. The results demonstrate that antigen delivery to different targets on DCs in vivo gives rise to humoral responses that differ in strength. Targeting of antigen to CD11c, CD36, CD205, Clec6A, Clec7A and PDC-TREM induced significantly stronger antibody responses compared to non-targeted isotype-matched controls. Targeting of Clec9A and Siglec-H did not lead to efficient antibody responses, which may be due to unfavourable properties of the targeting antibody, in which case, other antibodies with the same specificity might elicit a different outcome. Anti-CD11c was additionally used for elucidating...Continue Reading

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·H WangP Ghazal
Mar 26, 2002·Nature Reviews. Immunology·Carl G FigdorGosse J Adema
Oct 2, 2003·Current Opinion in Cell Biology·Alessandra Cambi, Carl G Figdor
Mar 17, 2004·The Journal of Experimental Medicine·Laura C BonifazRalph M Steinman
Jan 19, 2006·Annals of Oncology : Official Journal of the European Society for Medical Oncology·D SchadendorfUNKNOWN DC study group of the DeCOG
Mar 1, 2006·The Journal of Experimental Medicine·Silvia B BoscardinMichel C Nussenzweig
May 9, 2006·Trends in Immunology·Amanda L Blasius, Marco Colonna
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert W CarterDavid F Tough
Mar 24, 2007·Nature Reviews. Immunology·Paul R CrockerAjit Varki
Sep 15, 2007·Nature Reviews. Immunology·Paul J TackenCarl G Figdor
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi WataraiMasaru Taniguchi
May 24, 2008·The Journal of Clinical Investigation·David SanchoCaetano Reis e Sousa
Jul 25, 2008·European Journal of Immunology·Fernanda V V CastroMartin J Glennie
Jan 13, 2009·Molecular Immunology·Rachel E ShrimptonMary A Ritter
Feb 7, 2009·European Journal of Immunology·Irina CaminschiKen Shortman
Feb 24, 2009·Current Opinion in Immunology·Jill W Ford, Daniel W McVicar
Mar 17, 2009·Experimental & Molecular Medicine·Ken ShortmanIrina Caminschi
May 28, 2009·Science Signaling·Roy L Silverstein, Maria Febbraio
Jun 13, 2009·Nature Reviews. Immunology·Teunis B H Geijtenbeek, Sonja I Gringhuis
Jul 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Huafeng WeiYajun Guo
Mar 25, 2010·European Journal of Immunology·Olivier P JoffreCaetano Reis e Sousa
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Juliana IdoyagaRalph M Steinman
Mar 1, 2011·Physiology & Behavior·Céline MartinPhilippe Besnard
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mireille H LahoudIrina Caminschi
Nov 16, 2011·The Journal of Experimental Medicine·Melissa SwieckiMarco Colonna
Mar 23, 2012·Nature Reviews. Cancer·Karolina Palucka, Jacques Banchereau
Apr 10, 2012·Immunity·Jian-Guo ZhangMireille H Lahoud
May 9, 2012·Frontiers in Immunology·Irina CaminschiWilliam Ross Heath
Jun 26, 2013·Cellular Microbiology·Kate E KillickJames Harris
Aug 13, 2013·PLoS Pathogens·Rebecca A Drummond, Gordon D Brown
Apr 18, 2014·Science Translational Medicine·Madhav V DhodapkarTibor Keler
Jun 10, 2014·Frontiers in Immunology·Lillian Cohn, Lélia Delamarre

❮ Previous
Next ❯

Citations

May 25, 2016·Clinical & Translational Immunology·Christophe MacriJustine D Mintern
Feb 24, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arnar GudjonssonEven Fossum
Dec 1, 2017·Journal of Immunotherapy·Marlene F LaursenEmil Kofod-Olsen
Sep 29, 2019·Cancer Immunology, Immunotherapy : CII·Marlene Fyrstenberg LaursenRalf Agger
Jun 4, 2019·Frontiers in Immunology·Arnar GudjonssonEven Fossum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.