PMID: 9801430Nov 5, 1998Paper

Enhanced immune response after subcutaneous and oral immunization with biodegradable PLGA microspheres

Journal of Controlled Release : Official Journal of the Controlled Release Society
M IgartuaJ L Pedraz

Abstract

PLGA microspheres containing bovine serum albumin (BSA) as a model antigen, were prepared by a double emulsion/solvent extraction method and their in vitro characterization was performed. The same microspheres were used in a series of in vivo studies to evaluate the immune response induced after subcutaneous or oral inoculation following different immunization protocols. The in vivo data confirm that the immunogenicity of the albumin is not affected by the encapsulation procedure. The subcutaneous administration of microspheres showed an immune response (serum IgG levels by ELISA) statistically above BSA solution, even when the dose administered was 10 times lower. The adjuvanticity of the microspheres was found to be comparable to that of Freund's complete adjuvant (FCA), but in contrast to FCA they are biocompatible and did not induce any adverse reaction at the site of injection. A single oral administration of the microspheres was not a successful strategy for the induction of a reproducible response. Therefore, microspheres of 1 and 5 micrometer were orally administered on 3 consecutive days and the response obtained showed that the use of a boosting dose was not necessary for the 1 micrometer particles. These results sugg...Continue Reading

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Related Concepts

LactoSorb
Immunologic Adjuvants
Hemocompatible Materials
Enzyme-Linked Immunosorbent Assay
Freund's Adjuvant
Polyglobin
Variolation
Subcutaneous Injections
Mice, Inbred BALB C
Latex Spheres

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