Enhanced immunity against hepatoma induced by dendritic cells pulsed with Hsp70-H22 peptide complexes and CD40L.

Journal of Cancer Research and Clinical Oncology
Jian GaoTao Deng

Abstract

Dendritic cell (DC)-based cancer vaccines have become an attractive antitumour therapeutic approach. However, clinical application of current DC-based cancer vaccines has been limited by their ineffectiveness. Heat shock protein 70 from Mycobacterium tuberculosis (TBhsp70) is known to have a potent adjuvant capability to induce maturation of DCs and thus acts as an alternative ligand to the CD40 ligand (CD40L) on T cells to induce a T-cell response. The aim of this study is to investigate whether the combination of TBhsp70-H22 tumour-peptide complexes and CD40L might improve the antitumour efficacy for development of therapeutic DC-based vaccines against hepatoma. The CD40, CD80, CD86 and HLA-DR expression on DCs pulsed with TBhsp70-H22 tumour-peptide complexes and soluble CD40L was studied by flow cytometric analysis, and T-helper type 1 cytokine secretion, such as IL-12p70 secretion, was tested by ELISA. The H22-specific cytotoxic T-lymphocytes (CTLs) were detected by a (51)Cr-release assay, and the in vivo antitumour immunity against hepatoma was measured by utilising H22-tumour-bearing mice after therapeutic administration. Up-regulation of CD40, CD80, CD86 and HLA-DR expression on DCs pulsed with TBhsp70-H22 tumour-peptide...Continue Reading

References

Oct 1, 1994·The Journal of Experimental Medicine·C CauxJ Banchereau
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Mar 23, 2001·Nature Immunology·M Moser, K M Murphy
Feb 13, 2001·Molecular Immunology·A B AlexandroffD D Taub
Mar 22, 2002·The Lancet Oncology·M JeffordI D Davis
Jun 27, 2002·British Journal of Cancer·G ReinhardI G H Schmidt-Wolf
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yufei WangThomas Lehner
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Michael R ShurinGalina V Shurin
Oct 3, 2002·The Journal of Cell Biology·Thalia BeckerFelix Wieland
Nov 25, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leon T van den BroekeJay A Berzofsky
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Theresa L WhitesideSoldano Ferrone
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yufei WangThomas Lehner
Aug 4, 2005·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·E C H Lai, W Y Lau
Dec 23, 2006·Cancer Immunology, Immunotherapy : CII·Ying-Hua LanHong Ren
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sharon CohenNurit Hollander

❮ Previous
Next ❯

Citations

Sep 20, 2018·International Journal of Molecular Sciences·Sungmin LeeBuHyun Youn
Jul 9, 2020·Cancers·Estela Noguera-OrtegaEsther Julián

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.