Enhanced immunogenicity of a T cell immunogenic peptide by modifications of its N and C termini

International Immunology
P M AllenE R Unanue

Abstract

The modification of the terminal ionizable charges of an immunogenic peptide, HEL (46-61), was found to greatly increase the immunogenicity of the peptide. The modified peptide had 100- to 1000-fold enhanced activity in both in vitro and in vivo T cell assays. The mechanism of the enhancement was investigated by determining the binding affinities to I-Ak as well as circular dichroism (CD) studies. The native and enhanced peptides had indistinguishable binding affinities, as well as similar kinetics. The CD studies revealed that in aqueous solution, neither peptide had any detectable helicity; however, the addition of trifluoroethanol did result in significant helicity; with the two peptides being indistinguishable. These same modifications were also shown to enhance other immunogenic peptides if they contained a basic carboxy-terminal amino acid residue. Thus, by modifying the termini of T cell epitopes, their immunogenicity can be dramatically increased, but the molecular basis for this enhancement is still unclear.

Citations

Nov 6, 1990·Journal of Immunological Methods·R Busch, J B Rothbard
Jun 24, 1994·Journal of Immunological Methods·J C ReeceS J Rodda
Jun 10, 1996·Journal of Immunological Methods·T C ManningD M Kranz
Feb 1, 1991·Current Opinion in Immunology·C V Harding
Jul 24, 2002·Journal of Immunological Methods·Stuart J Rodda
Jul 31, 1998·Molecular Immunology·K A WilkinsonF Hudecz
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R W RoofE R Unanue
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C A NelsonE R Unanue
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·K Mason, H M McConnell
Nov 26, 1999·The Journal of General Virology·Y GaoG A Splitter
Dec 13, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hernando CurtidorManuel E Patarroyo
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·X DongR D Salter
Aug 5, 2015·Expert Opinion on Biological Therapy·Hernando CurtidorManuel A Patarroyo

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