Enhanced immunogenicity of the modified GP5 of porcine reproductive and respiratory syndrome virus

Virus Genes
Liurong FangHuanchun Chen

Abstract

The ORF5-encoded major envelope glycoprotein (GP5) is one of the key immunogenic proteins of the porcine reproductive and respiratory syndrome virus (PRRSV) and is the leading target for the development of the new generation of vaccines against PRRS. However, weak and tardy neutralizing antibodies have been elicited in several developed experimental vaccines expressing PRRSV GP5. More recent evidence has demonstrated a non-neutralizing decoy epitope upstream of the neutralizing epitope of GP5, which might prevent the development of a strong neutralizing antibody response against PRRSV. In the present study, we modified the ORF5 gene by inserting a Pan DR T-helper cell epitope (PADRE) between the neutralizing epitope and the decoy epitope to minimize or eliminate the decoy effect of the non-neutralizing epitope. The immunogenicity of the modified GP5 was further evaluated using DNA vaccination. The results showed that significantly enhanced neutralizing antibodies were elicited in mice immunized with the DNA construct expressing the modified GP5 compared with the native GP5. Slightly increased levels of GP5-specific ELISA antibodies and T-cell proliferative activities were also observed. These results indicate that the high immu...Continue Reading

References

Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
Jul 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·I J YoonT W Molitor
Dec 9, 1998·Immunologic Research·J AlexanderA Sette
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J AlexanderA Sette
Jan 7, 2003·Viral Immunology·Michael P MurtaughFederico Zuckermann
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura A Novotny, Lauren O Bakaletz
Sep 27, 2003·British Journal of Haematology·Enrico CrivellatoDomenico Ribatti
Aug 19, 2004·Vaccine·Annette Malene BarfoedSøren Kamstrup

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Citations

Jan 26, 2012·Journal of Virological Methods·Yuanyuan WangHuanchun Chen
May 26, 2009·Vaccine·Tjeerd G KimmanNorbert Stockhofe-Zurwieden
Jan 27, 2009·Biochemical and Biophysical Research Communications·Yuanyuan WangHuanchun Chen
Jul 24, 2007·The Veterinary Journal·E Mateu, I Diaz
Jun 23, 2007·Journal of Fish Diseases·J Z CostaR H Richards
Mar 13, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xiaomin WangZhengyu Yu
May 1, 2013·Journal of Veterinary Science·Eun-Jin ChoiYeun-Kyung Shin
Jan 9, 2017·Veterinary Immunology and Immunopathology·Luping DuKehe Huang

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