Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture.

Plant Cell Reports
M SorianoA M Castillo

Abstract

The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in the induction media for 5 h, highly improved embryo production in both cultivars. Sec- and tert-butanol performed similarly to control plates. Regeneration ability was unaffected by any butyl-alcohol treatment. As a consequence of the higher embryo production after n-butanol treatment, the number of green regenerated plants increased up to five times in cultivar Pavon and up to three times in cultivar Caramba. The percentage of green plants was improved or unaffected by the treatment. Doubled haploid plant production was between 2 and 4 times higher after n-butanol treatment than in control plates. Therefore, n-butanol was successfully applied in the production of wheat doubled haploids. This primary alcohol is known as an activator of phospholipase D and has been previously reported to disrupt cortical microtubules and detach them from the plasma membrane in plants. Its effects on androgenetic induction could confir...Continue Reading

References

Dec 6, 2001·Science's STKE : Signal Transduction Knowledge Environment·T Munnik, A Musgrave
Aug 16, 2002·Current Opinion in Plant Biology·Ana M Laxalt, Teun Munnik
Dec 1, 1995·The Plant Cell·T. MunnikA. Musgrave
Jan 27, 2004·The Journal of Biological Chemistry·Laurent ThieryArnould Savouré
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wenhua ZhangXuemin Wang
Apr 1, 2006·Progress in Lipid Research·Xuemin WangRuth Welti
May 16, 2006·Plant & Cell Physiology·Ai HiraseSeiji Sonobe
Jun 13, 2006·Phytochemistry·Flavia Navari-IzzoMike F Quartacci
Aug 1, 2006·Current Opinion in Plant Biology·Bastiaan O Bargmann, Teun Munnik
May 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B BarnabásG Kovács

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Citations

Jul 16, 2013·Plant Reproduction·Mercedes SorianoKim Boutilier
Jul 11, 2013·Plant Reproduction·Rosa Angélica Sánchez-DíazMaría Pilar Vallés
Mar 18, 2010·Plant Biotechnology Journal·Jim M Dunwell
Aug 26, 2009·Plant Signaling & Behavior·Jianping Hu, Mintu Desai

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