Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species

Virology
P P MertensP S Mellor

Abstract

Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a cell line (KC cells) derived from a species of Culicoides that can act as a vector for BTV (Culicoides variipennis), demonstrated a much higher infectivity of core particles than that in mammalian cells (approximately x 10(3)). This increase resulted in a specific infectivity for cores that was only 20-fold less than that of purified disaggregated virus particles (stored in the presence of 0.1% sodium-N-lauroylsarcosine (NLS)). Removal of this detergent caused intact virus particle aggregation and (as previously reported) resulted in an approximately 1 log10 drop in the specific infectivity of those virus particles which remained in suspension. In consequence the specific infectivity of core particles for the KC cells was directly comparable to that of the intact but aggregated virus. These data are compared with the results from oral infectivity studies using two vector species (C. variipennis and Culicoides nubec...Continue Reading

Citations

Aug 13, 2010·Journal of Virology·Cheng-Qiang HeHong-Shan Guo
Dec 28, 2012·Journal of Virology·Esther SchnettlerAlain Kohl
Jun 23, 2000·Journal of Virology·E L NasonB V Venkataram Prasad
Mar 1, 2013·Parasites & Vectors·Leonardo Saboia-VahiaJose B De Jesus
Dec 3, 2008·Emerging Infectious Diseases·Martin A HofmannBarbara Thuer
Sep 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Andrew R BeatonPolly Roy
Oct 24, 2000·Journal of Comparative Pathology·P S Mellor
Apr 5, 2011·Research in Veterinary Science·Jeannette PlanzerBarbara Thür
Sep 18, 2002·Archives of Medical Research·William C BlackBarry J Beaty
Apr 16, 1999·Journal of Virological Methods·C L HillP P Mertens
Aug 29, 2003·Journal of Medical Entomology·Linda E McHolland, James O Mecham
Feb 16, 2010·Veterinary Microbiology·Gabriella WorwaBarbara Thür
Aug 29, 2009·Veterinary Microbiology·I M G A Santman-BerendsP A van Rijn
Jan 24, 2007·Journal of Virological Methods·S AnthonyP P C Mertens
Nov 16, 2012·The Journal of General Virology·D LegisaM J Dus Santos
Dec 29, 2013·Virus Research·Avnish Patel, Polly Roy
Jan 1, 1997·Annual Review of Immunology·J J DonnellyM A Liu
May 23, 2008·Veterinary Research·Isabelle Schwartz-CornilStéphan Zientara
Oct 17, 1998·Nature·J M GrimesD I Stuart
Dec 3, 2016·Journal of Virology·René G P van GennipPiet A van Rijn
Dec 20, 2008·Veterinary Research·Anthony WilsonPeter Paul Clement Mertens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.