Enhanced intracellular calcium induced by urocortin is involved in degranulation of rat lung mast cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yuqing WuShengnan Li

Abstract

Corticotropin-releasing factor (CRF), which activates the hypothalamic-pituitary- adrenal axis under stress, also has proinflammatory peripheral effects possibly through mast cells. The purpose of this study was to investigate the effect of urocortin (UCN), a 40-amino-acid CRF family peptide, on degranulation and intracellular calcium of rat lung mast cells. The activation and degranulation of mast cells were observed by Toluidine blue staining and transmission electron microscope. The intracellular calcium was investigated using confocal laser scanning microscopy and flow cytometry. The results indicated that all the three different concentrations of UCN (0.1, 1 and 10 microM) significantly induced the activation and degranulation of rat lung mast cells in vitro. This effect was markedly blocked by selective CRF receptor 1 (CRF-R1) antagonist antalarmin, but not by specific CRF receptor 2 (CRF-R2) antagonist antisauvagine-30 (anti-Svg-30). The results also showed that UCN caused a rapid peak increase in [Ca(2+)](i) at point of 300s after UCN treatment, followed by a decrease to a sustained plateau phase. The peak increase in [Ca(2+)](i) induced by UCN was significantly inhibited by antalarmin, but not by anti-Svg-30. This effe...Continue Reading

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Citations

Apr 9, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Huayuan ZhuShengnan Li
Mar 19, 2009·Biochemical and Biophysical Research Communications·Rongjian Zhang, Shengnan Li
Apr 10, 2012·Psychoneuroendocrinology·Timothy G Dinan, John F Cryan
Jul 8, 2011·Neuroendocrinology·Luciana Souza-MoreiraElena Gonzalez-Rey
Jul 24, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Santiago Balseiro-GomezEva Ales
Feb 28, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J ManningD O'Malley
Apr 24, 2019·Nutrients·Luis G Bermúdez-HumaránOscar K Bitzer-Quintero
Apr 25, 2009·Current Opinion in Endocrinology, Diabetes, and Obesity

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