Enhanced intracellular delivery via coordinated acoustically driven shear mechanoporation and electrophoretic insertion

Scientific Reports
J Mark MeachamAndrei G Fedorov

Abstract

Delivery of large and structurally complex target molecules into cells is vital to the emerging areas of cellular modification and molecular therapy. Inadequacy of prevailing in vivo (viral) and in vitro (liposomal) gene transfer methods for delivery of proteins and a growing diversity of synthetic nanomaterials has encouraged development of alternative physical approaches. Efficacy of injury/diffusion-based delivery via shear mechanoporation is largely insensitive to cell type and target molecule; however, enhanced flexibility is typically accompanied by reduced gene transfer effectiveness. We detail a method to improve transfection efficiency through coordinated mechanical disruption of the cell membrane and electrophoretic insertion of DNA to the cell interior. An array of micromachined nozzles focuses ultrasonic pressure waves, creating a high-shear environment that promotes transient pore formation in membranes of transmitted cells. Acoustic Shear Poration (ASP) allows passive cytoplasmic delivery of small to large nongene macromolecules into established and primary cells at greater than 75% efficiency. Addition of an electrophoretic action enables active transport of target DNA molecules to substantially augment transfect...Continue Reading

References

Mar 1, 1990·Biochimica Et Biophysica Acta·D S Dimitrov, A E Sowers
Mar 23, 1990·Science·J A WolffP L Felgner
Apr 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H LambertR Hancock
Feb 18, 1972·Science·S J Singer, G L Nicolson
Jan 15, 2000·The Journal of Biological Chemistry·G L LukacsA S Verkman
Jun 27, 2000·Biophysical Journal·K OlbrichE Evans
Mar 7, 2001·Physics in Medicine and Biology·M Lokhandwalla, B Sturtevant
Jan 17, 2003·The New England Journal of Medicine·Salima Hacein-Bey-AbinaAlain Fischer
Sep 26, 2003·Biophysical Journal·Evan EvansWieslawa Rawicz
Sep 14, 2004·Biophysical Journal·J K ArmstrongT C Fisher
Feb 22, 2005·Advanced Drug Delivery Reviews·Mattias BeltingAnders Wittrup
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abdelatif Elouahabi, Jean-Marie Ruysschaert
Mar 11, 2005·Advanced Drug Delivery Reviews·Delphine LechardeurGergely L Lukacs
Jul 13, 2006·Annual Review of Biomedical Engineering·Vladimir P Torchilin
Oct 24, 2006·Biophysical Journal·Wanda Krassowska, Petar D Filev
Jul 4, 2007·Nature Reviews. Genetics·Reinhard WaehlerDavid T Curiel
Sep 20, 2007·Biotechnology and Bioengineering·Daniel M HallowMark R Prausnitz
Nov 13, 2007·Biomedical Microdevices·Vladimir G ZarnitsynAndrei G Fedorov
Jun 3, 2008·Journal of Immunological Methods·Jonathan JantschAlexander T Prechtel
Aug 30, 2008·Current Opinion in Biotechnology·Yan Zhang, Long-Chuan Yu
Mar 25, 2009·British Journal of Pharmacology·Frederic HeitzGilles Divita
Dec 17, 2009·Journal of Immunological Methods·Melanie WieseJonathan Jantsch
Jul 20, 2010·Nature Nanotechnology·Prerona ChakravartyMark R Prausnitz
Jan 21, 2011·Analytical Chemistry·Ting-Hsiang WuPei-Yu Chiou
Jun 14, 2012·Stem Cells International·Marc ThierFrank Edenhofer
Jan 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Armon ShareiKlavs F Jensen
Jul 3, 2013·Journal of Laboratory Automation·J Mark MeachamAndrei G Fedorov
Feb 27, 2014·Cellular Reprogramming·Suresh RamakrishnaKwang-Hyun Baek
May 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·M HyodoH Harashima
Apr 16, 2015·PloS One·Armon ShareiKlavs F Jensen
May 23, 2015·Scientific Reports·Gregory Lee SzetoDarrell J Irvine
Oct 14, 2016·Nature·Martin P StewartKlavs F Jensen

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Citations

Jul 27, 2018·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Andrew D LedbetterJ Mark Meacham
Aug 28, 2020·Advanced Materials·Yaping ChenRoey Elnathan
Mar 3, 2019·Scientific Reports·Justin A JarrellRyan S Pawell
Feb 18, 2021·Advanced Materials·Dorsa Morshedi RadMajid Ebrahimi Warkiani
May 2, 2021·Biotechnology Advances·Laurens RaesKevin Braeckmans
May 21, 2021·Cell Transplantation·Lauren M TimminsBiju Parekkadan
Oct 28, 2019·Nano Letters·Harish G DixitMasaru P Rao
Jul 28, 2018·Chemical Reviews·Martin P StewartKlavs F Jensen
Sep 3, 2021·The Journal of the Acoustical Society of America·Li ShanJ Mark Meacham

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Methods Mentioned

BETA
transfection
electrophoresis
fluorescence microscopy
flow cytometry
scanning electron microscopy
flow
fluorescence
scanning

Software Mentioned

ASP
Flowing
FACSDiva
BD

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