Enhanced isolation of Serpulina hyodysenteriae by using sliced agar media.

Journal of Clinical Microbiology
L D Olson

Abstract

A method has been developed for separating Serpulina hyodysenteriae, a large spirochete and the causative agent of swine dysentery (SD), from other fecal anaerobic bacteria in rectal and colonic swabs. This was done by cutting the blood agar in parallel cuts and streaking perpendicular to the cuts in the center of the petri dish. Migration of S. hyodysenteriae from the central streak was apparent by the presence of strong beta-hemolysis along the edges of the cuts. If only S. hyodysenteriae migrated in the cut, they migrated to the end of the cut. However, if both motile bacteria and S. hyodysenteriae migrated in the cut, the motile bacteria migrated to the end of the cut where they formed colonies and the S. hyodysenteriae located along the edges of the cut between the colonies of motile bacteria and the central streak. Although motile bacteria were present where S. hyodysenteriae located, the growth of the motile bacteria was partially inhibited since they rarely formed visible colonies and were low in number. The cut in the agar was thought to improve traction for the serpentine movement of the S. hyodysenteriae and for the flagellar movement of the motile bacteria. Use of sliced blood agar was superior to conventionally str...Continue Reading

References

Nov 1, 1988·Journal of Clinical Microbiology·R A Kunkle, J M Kinyon
Nov 1, 1971·The British Veterinary Journal·D J Taylor, T J Alexander
Oct 1, 1983·Journal of Clinical Microbiology·L D Olson, W H Fales

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Citations

Sep 15, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Friederike ZeehHeiko Nathues

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