Enhanced L-methionine production by genetically engineered Escherichia coli through fermentation optimization

3 Biotech
Hai-Yan ZhouYu-Guo Zheng

Abstract

Microbial fermentation for L-methionine (L-Met) production based on natural renewable resources is attractive and challenging. In this work, the effects of medium composition and fermentation conditions were investigated to improve L-Met production by genetically engineered Escherichia coli MET-3. Statistical optimization techniques including Plackett-Burman (PB) design and Box-Behnken design (BBD) were adopted first to optimize the culture medium. Results of PB-designed experiments indicated that the culture medium components including glucose, yeast extract, KH2PO4, and MgSO4.7H2O had significant effects on L-Met biosynthesis. With their best-predicted concentration established by BBD (glucose 37.43 g/L, yeast extract 0.95 g/L, KH2PO4 1.82 g/L, and MgSO4.7H2O 4.51 g/L), L-Met titer was increased to 3.04 g/L from less than 2.0 g/L. For further enhancement of L-Met biosynthesis, the fermentation conditions of batch cultivation carried out in a 5-L fermentor were optimized, and the optimum results were obtained at an agitation rate of 300 rpm, medium pH of 7.0, and induction temperature of 28 °C. Based on the optimization parameters, fed-batch fermentation with the modified medium was conducted. As a result, great improvement of...Continue Reading

References

Jul 1, 1991·Molecular Microbiology·H Weissbach, N Brot
Jan 1, 1996·Folia Microbiologica·S MondalS P Chatterjee
Jun 4, 2005·Applied and Environmental Microbiology·Yoshihiro Usuda, Osamu Kurahashi
Jul 3, 2007·Metabolic Engineering·Soo-Dong ParkHeung-Shick Lee
Jun 19, 2008·Journal of Industrial Microbiology & Biotechnology·Juliano Lemos BicasGláucia Maria Pastore
Dec 30, 2009·International Journal of Pharmaceutics·Ziyaur RahmanMansoor A Khan
Oct 30, 2010·Journal of Experimental Botany·Martin A J ParryRobert T Furbank
Sep 18, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoli ZhangHaixia Zhang
Sep 17, 2014·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Ghazal LabbeikiAlimorad Rashidi
Nov 9, 2014·Applied Microbiology and Biotechnology·Thomas Willke
Oct 22, 2016·Biotechnology and Bioengineering·Jian-Feng HuangYu-Guo Zheng
Nov 16, 2016·Journal of Industrial Microbiology & Biotechnology·Hua LiGui Yang Shi
Jan 12, 2017·Applied Microbiology and Biotechnology·Yu-Guo ZhengZhi-Qiang Liu

❮ Previous
Next ❯

Citations

May 20, 2020·Frontiers in Bioengineering and Biotechnology·Hai-Yan ZhouYu-Guo Zheng
Sep 27, 2020·Biotechnology and Applied Biochemistry·Shu-Ping ZouYu-Guo Zheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.