Enhanced light microscopic visualization of oxidase activity with the cerium capture method.

Histochemistry
R GossrauW M Frederiks

Abstract

Visualization methods for the light microscopic detection of the activity of oxidases after being localized with cerium ions as reported by Angermüller and Fahimi (1988a, b) are not suitable for the demonstration of H2O2-genrating oxidases at sites with low activity. Therefore, the cerium-diaminobenzidine (DAB) visualization procedure of these authors was modified. Nickel or cobalt ions were added to the DAB solution together with small amounts of H2O2. Visualization was performed in a one-step-method. This modified visualization technique enables light microscopic detection of amino acid oxidase activity in kidney and liver cells where it was found with the original method but the amounts of final reaction product were considerably higher. Moreover, the DAB-nickel-H2O2 and DAB-cobalt-H2O2 procedures were more sensitive than the cerium-lead method of Angermüller and Fahimi (1988a, b). The method appeared to be specific, because final reaction product was not found after control incubation. Especially the DAB-nickel-H2O2 procedure can also be used for immunohistochemistry when glucose oxidase serves as the enzyme label.

References

Jan 1, 1979·Progress in Histochemistry and Cytochemistry·J S Hanker
Jan 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Angermüller, H D Fahimi
Jun 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W D Kuhlmann, S Avrameas
Oct 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M Hsu, E Soban
Jul 1, 1958·Clinica Chimica Acta; International Journal of Clinical Chemistry·F FEIGL, E JUNGREIS

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Citations

May 8, 1998·Cell Biology International·I Cancio, M P Cajaraville
Jan 1, 1990·Histochemistry·R Gossrau, H Franz
Jan 1, 1993·Journal of Inherited Metabolic Disease·W M FrederiksR J Wanders
Dec 1, 1993·Acta histochemica·G Nakos, R Gossrau
May 1, 1993·Acta histochemica·I H Straatsburg, R Gossrau
Feb 28, 2002·Free Radical Biology & Medicine·Minjie ZhangKiyohisa Sekizawa
Jan 1, 1997·Free Radical Biology & Medicine·W M Frederiks, K S Bosch
May 8, 2002·Acta histochemica·Wilma M Frederiks, Helena Vreeling-Sindelárová
Jul 1, 1993·Journal of Microscopy·C J Van Noorden, W M Frederiks
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·S V Khoronenkova, V I Tishkov
Jun 1, 1996·The Histochemical Journal·R J Van den Munckhof
Mar 1, 1990·The Histochemical Journal·R GossrauW M Frederiks
Aug 19, 2010·Chemical Society Reviews·Ajay KarakotiWilliam T Self
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·K J HalbhuberN Zimmermann
Jul 1, 1995·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·G Nakos, R Gossrau
Nov 1, 1995·The Histochemical Journal·R J Van Den MunckhofW M Frederiks
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H D Fahimi, E Baumgart

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