Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration

Lab on a Chip
Alexandre ChícharoPaulo P Freitas

Abstract

We report the design and characterization of a lateral and vertical hydrodynamic focusing feature for whole cell detection on a miniaturized flow cytometer. The developed system, based on magnetic sensing, incorporates spin valve sensors on the bottom of the microfluidic channels that detect cells labeled with magnetic beads. An adaptable 3D hydrodynamic focusing system was developed that pushes labeled cells towards the bottom of the microchannel, closer to the sensors, allowing increased signal amplitude for cells labeled with magnetic beads and enhanced discrimination of labeled cells. Fluorescence microscopy indicates that the lateral and vertical hydrodynamic focusing effect was adequately implemented, consistent with simulation predictions. The sensitivity of the system to detect labeled cells was improved by at least two-fold. By estimating the coverage of magnetic beads on cells, the signal from labeled cells could be predicted using a mathematical model, which also demonstrated the sensitivity of the signal to the height of the cells relative to the sensor. The system is versatile allowing interchangeable flow rates for cells with different diameters.

References

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Nov 26, 2009·Biosensors & Bioelectronics·Mansoor NasirFrances S Ligler
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Apr 14, 2016·The Analyst·Rachael SmithLorna Ashton

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Citations

Sep 5, 2019·Biosensors·Manon GiraudGuénaëlle Jasmin-Lebras
Feb 21, 2019·Analytical and Bioanalytical Chemistry·Rita SoaresPaulo P Freitas

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Methods Mentioned

BETA
light scattering
chip
optical microscopy
Fluorescence
confocal microscopy
fluorescence microscopy

Software Mentioned

COMSOL Multiphysics
MatLab

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