Enhanced method performances for conventional and chiral CE-ESI/MS analyses in plasma

Electrophoresis
Julie SchapplerS Rudaz

Abstract

Due to its high efficiency, selectivity, and sensitivity, CE-ESI/MS has evolved as an efficient technique for the drugs and metabolites analysis in biological matrices. However, a sample preparation is mandatory prior to CE-ESI/MS analysis. To achieve fast and simplified sample preparation of plasma samples, protein precipitation (PP) and liquid-liquid extraction (LLE) were used with two injection techniques: hydrodynamic (HD) and electrokinetic (EK) injection. CE-ESI/MS analyses of pharmaceutical compounds and amphetamine derivatives were developed. Detection limits of 1 ppm were reached with PP and HD injection whereas 1 ppb was detected when samples were prepared with LLE and injected by EK. Same experiments were performed for stereoselective determinations in partial-filling mode and detection limits achieved were equivalent to conventional analysis (0.5 ppb per enantiomer). When complex matrices are analyzed, MS signal suppression or enhancement effects are generally not reproducible and could compromise results with ESI. Therefore, matrix effect was investigated in CE-ESI/MS with a commercially available coaxial sheath-liquid ESI interface used as postcapillary infusion system to determine MS signal alterations. Matrix ef...Continue Reading

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Citations

Apr 13, 2012·Journal of Pharmaceutical and Biomedical Analysis·Isabelle KohlerSerge Rudaz
Feb 26, 2010·Journal of Chromatography. a·Govert W SomsenGerhardus J de Jong
Aug 5, 2009·Electrophoresis·Peter Mikus, Katarína Maráková
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Dec 7, 2007·Electrophoresis·Franco Tagliaro, Federica Bortolotti
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin
Dec 29, 2007·Electrophoresis·Laura Sánchez-HernándezCarmen García-Ruiz
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Apr 9, 2010·Electrophoresis·Carolina SimóAlejandro Cifuentes

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