Enhanced mRNA cap methylation increases cyclin D1 expression and promotes cell transformation.

Oncogene
Victoria H Cowling

Abstract

Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNA guanine-7-methyltransferase (RNMT). mRNA cap methylation was recently described to be rate-limiting for a subset of mRNAs, and to be enhanced by expression of c-Myc and E2F1, although the biological significance of this finding was not investigated. Here, it is reported that increased RNMT expression enhances cellular mRNA cap methyltransferase activity, promotes mammary epithelial cell transformation and cooperates with H-RasV12 or c-Myc to promote fibroblast cell transformation. Cyclin D1 is a prominent oncogene in epithelial tumours. A significant fraction of Cyclin D1 mRNA was found to be unmethylated on the mRNA cap and thus dormant in mammary epithelial cells. Cyclin D1 expression was increased by enhanced mRNA cap methylation. In summary, this report shows that mRNA cap methylation is rate-limiting for expression of an oncogene and cell transformation.

References

Dec 1, 1976·Cell·A J Shatkin
Jun 1, 1994·Molecular and Cellular Biology·A PhilippM Eilers
Aug 15, 1998·The Journal of Biological Chemistry·R C PillutlaA J Shatkin
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Oct 29, 2000·Advances in Virus Research·Y Furuichi, A J Shatkin
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Stewart Shuman
Mar 19, 2004·Nature·Hans-Guido WendelScott W Lowe
Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
May 20, 2005·Current Opinion in Cell Biology·David L Bentley
May 6, 2006·The Breast : Official Journal of the European Society of Mastology·Pankaj G Roy, Alastair M Thompson
May 18, 2006·Molecular and Cellular Biology·Victoria H CowlingMichael D Cole
May 9, 2007·Molecular and Cellular Biology·Victoria H CowlingMichael D Cole
Dec 7, 2007·Genes & Development·Hans-Guido WendelScott W Lowe
Aug 14, 2008·Nature Reviews. Molecular Cell Biology·Michael D Cole, Victoria H Cowling
Nov 5, 2008·Trends in Genetics : TIG·Robert J White
Nov 22, 2008·FEBS Letters·Ya-Ping TsaiKou-Juey Wu

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Citations

Mar 23, 2012·Nature Reviews. Cancer·Andrew M ScottLloyd J Old
Mar 5, 2016·Molecular Cell·Michael AreggerVictoria H Cowling
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Ivan TopisirovicAaron J Shatkin
May 18, 2012·Cell Cycle·Michael Aregger, Victoria H Cowling
Nov 22, 2011·Molecular Cell·Thomas Gonatopoulos-PournatzisVictoria H Cowling
Jul 17, 2016·Nucleic Acids Research·Dhaval VarshneyVictoria H Cowling
Apr 13, 2018·Bio-protocol·Jackson B Trotman, Daniel R Schoenberg
Oct 21, 2016·Oncotarget·Olivia LombardiVictoria H Cowling
Jun 5, 2019·Cold Spring Harbor Perspectives in Biology·Bastian Linder, Samie R Jaffrey
Sep 17, 2020·Critical Reviews in Biochemistry and Molecular Biology·Federica AccorneroJuan D Alfonzo
Dec 11, 2020·Wiley Interdisciplinary Reviews. RNA·Jerry PelletierNahum Sonenberg
Apr 26, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Barbara StefanskaMoshe Szyf
Oct 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Biljana Culjkovic-KraljacicKatherine L B Borden
Jan 13, 2021·International Journal of Molecular Sciences·Ligia I TorsinGeorge A Calin
Feb 20, 2021·Molecular Cell·Radha MukherjeeNeal Rosen
Jul 1, 2021·Nature Reviews. Cancer·Corey LourencoLinda Z Penn
Mar 20, 2018··Jackson TrotmanDaniel Schoenberg

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