PMID: 2117250Aug 1, 1990Paper

Enhanced mutability at the tk locus in the radiosensitive double-strand break repair mutant xrs5

Mutation Research
T A MussaP E Bryant

Abstract

The thymidine kinase locus (tk) has been utilised as the target locus to measure the induced mutation frequency following X-irradiation in the X-ray-sensitive xrs5 mutant and its parent CHO K1 line of Chinese hamster cells. Mutations to tk- cells were measured by plating cells in selective medium containing trifluorothymidine after a post-irradiation expression time of 4 days. Our results show that the mutation frequency was 3-4 times higher in the xrs5 mutant than in the CHO K1 cell line. This enhanced mutation frequency in xrs5 is though to result from the deficiency in DNA double-strand break repair in this cell line which also results in the enhanced cell killing and higher frequencies of chromosomal aberrations in response to X-irradiation. The findings of the present study suggest that DNA double-strand break is a critical lesion leading to mutations in irradiated cells.

References

Dec 23, 1976·Nature·P J HastingsR C von Borstel
Nov 1, 1988·Mutation Research·M Z ZdzienickaJ W Simons
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H H EvansJ Hozier
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H H EvansM F Horng
Jun 1, 1986·Mutation Research·M M MooreC Doerr
Oct 8, 1965·Biochemical and Biophysical Research Communications·J F Speyer
May 1, 1983·Cell·J W SzostakF W Stahl
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P KavathasR DeMars

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