Enhanced Na+-dependent bile salt uptake by WIF-B cells, a rat hepatoma hybrid cell line, following growth in the presence of a physiological bile salt

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
E M KonieczkoJ M Crawford

Abstract

Although bile salts are toxic to the liver at high plasma concentrations, the effects of physiological concentrations of bile salts on normal hepatic function are poorly understood. We examined the effect of taurocholate (TC) on the basolateral uptake of [3H]TC in WIF-B cells, a hybrid cell line stably exhibiting in vitro the structural and functional polarity of hepatocytes. Cells were grown in the absence or presence of TC (50 micromol/L) over 12 days, and then incubated with [3H]TC concentrations ranging from 1 to 250 micromol/L. For both control and TC-grown cells, uptake of [3H]TC was linear over 2 minutes. In control cells, the Km for [3H]TC Na+-dependent uptake over 1 minute was 6 +/- 5 micromol/L, and the Vmax was 45 +/- 6 pmol TC/mg protein/min (+/- SEM). TC-grown cells exhibited no significant change in Km but showed a doubling of Vmax to 87 +/- 6 pmol TC/mg protein/min (P < .005). In both control and TC-grown cells, maximal uptake of [3H]TC occurred following 10 to 12 days in culture, with TC-grown cells consistently showing greater rates of [3H]TC uptake from 4 to 14 days in culture. Western blots immunostained for the basolateral Na+-dependent plasma membrane protein, ntcp, revealed the appropriate approximately 50...Continue Reading

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Citations

Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J A DranoffM H Nathanson
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W N LianC H Lin
Jun 30, 1998·Current Opinion in Lipidology·M W Love, P A Dawson
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V BenderD Cassio
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E SturmJ M Crawford
Sep 8, 2000·Baillière's Best Practice & Research. Clinical Gastroenterology·P L Jansen
Jun 29, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Marcelo Borges CavalcanteRicardo Barini
Feb 10, 1999·The American Journal of Physiology·F R SimonE Sutherland

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