Enhanced Osteogenic Behavior of ADSCs Produced by Deproteinized Antler Cancellous Bone and Evidence for Involvement of ERK Signaling Pathway

Tissue Engineering. Part a
Jinqi WeiXuliang Deng

Abstract

Calcinated antler cancellous bone (CACB) is useful in repair of bone defects, as its composition and architecture is analogous to natural extracellular bone matrix. The use of CACB scaffolds with adipose-derived stem cells (ADSCs) in repair of rabbit mandibular bone defects was investigated along with the underlying molecular signaling pathways involved. CACB promoted the adhesion, spreading, and viability of ADSCs. Increased extracellular matrix production and expression of osteogenic markers in ADSCs were observed when seeded in CACB. The temporal kinetics of mRNA expression of ADSCs cultured in CACB lagged in comparison with that observed in cells grown in medium with osteogenic supplements. Activation of the extracellular signal-related kinases (ERK) 1/2 and RUNX-2 in CACB-cultured ADSCs was observed, and this activation was attenuated by the MeK inhibitor U0126. Microcomputed tomography scanning analysis and histological evaluations showed that loading the CACB with ADSCs resulted in enhanced new bone formation and angiogenesis when the composites were implanted in rabbit mandibular defects. These results indicated that the osteogenic behavior of ADSCs might be driven by the microenvironment formed by CACB via the ERK sign...Continue Reading

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Citations

Aug 16, 2016·Journal of Tissue Engineering and Regenerative Medicine·Siddharth ShanbhagAndreas Stavropoulos
Apr 12, 2017·Connective Tissue Research·Eric J TsangPatricia Zuk
Oct 6, 2018·Journal of Biomedical Materials Research. Part a·Xiaoman LiZhenzhong Yang
Apr 4, 2021·Materials·Andreea-Mariana Negrescu, Anisoara Cimpean
Jun 16, 2021·Research in Veterinary Science·Pierre P PicavetStéphanie Claeys

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Methods Mentioned

BETA
xenografts
xenograft
scanning electron microscopy
X-ray
infrared spectroscopy
Scanning Microscopy
Assay
PCR
light microscopy

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Quantity One
Image Pro Plus
Skyscan
Tomo NT

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