Enhanced Osteogenic Differentiation of Human Fetal Cartilage Rudiment Cells on Graphene Oxide-PLGA Hybrid Microparticles

Journal of Functional Biomaterials
Stuart C ThickettMegan S Lord

Abstract

Poly(d,l-lactide-co-glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering.

References

Aug 3, 2001·Blood Cells, Molecules & Diseases·M W Long
May 13, 2006·Journal of Biomaterials Science. Polymer Edition·Carlos E Astete, Cristina M Sabliov
Dec 25, 2008·Pharmaceutical Research·Christine Vauthier, Kawthar Bouchemal
Mar 31, 2009·Journal of Materials Science. Materials in Medicine·A Champa Jayasuriya, Nabil A Ebraheim
Jun 10, 2010·Journal of the American Chemical Society·Jaemyung KimJiaxing Huang
Aug 25, 2010·ACS Nano·Daniela C MarcanoJames M Tour
Nov 26, 2010·Journal of the American Chemical Society·Jiayan LuoJiaxing Huang
Jul 2, 2011·International Journal of Nanomedicine·R DinarvandF Atyabi
Nov 18, 2011·Colloids and Surfaces. B, Biointerfaces·Zengjiang WeiZhen Tong
Jul 11, 2012·European Cells & Materials·E M CzekanskaJ S Hayes
Mar 5, 2014·International Journal of Molecular Sciences·Piergiorgio GentilePaul V Hatton
Mar 15, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Megan A CreightonRobert H Hurt
Dec 19, 2014·Journal of Colloid and Interface Science·Stuart C Thickett, Per B Zetterlund
Feb 10, 2016·Tissue Engineering. Part B, Reviews·Aaron W JamesChia Soo
Jan 13, 2018·Nanoscale Research Letters·Chunyong LiangHongshui Wang
Mar 12, 2018·Materials Science & Engineering. C, Materials for Biological Applications·Dinesh Pratap SinghRajesh Kumar
Oct 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Megan S LordJohn M Whitelock

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Methods Mentioned

BETA
infrared spectroscopy
transmission electron microscopy
dynamic light scattering
PCR

Software Mentioned

DTS
ZetaSizer Nanoseries

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