Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells on Electrospun Polyethersulfone/Poly(Vinyl) Alcohol/Platelet Rich Plasma Nanofibrous Scaffolds

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Mahshid Sadat Kashef-SaberiHana Hanaee-Ahvaz

Abstract

Over the last few decades, great advancements have been achieved in the field of bone tissue engineering (BTE). Containing a great number of growth factors needed in the process of osteogenesis, platelet rich plasma (PRP) has gained a great deal of attention. However, due to the contradictory results achieved in different studies, its effectiveness remains a mystery. Therefore, in this study, we investigated in vitro performance of co-electrospun PRP/poly ether sulfone/poly(vinyl) alcohol (PRP/PES/PVA) composite scaffolds for the osteogenic differentiation of human adipose-derived mesenchymal stem cells. The activated PRP was mixed with PVA solution to be used alongside PES solution for the electrospinning process. Fourier transform infrared spectroscopy, scanning electron microscopy and tensile tests were performed to evaluate the scaffolds. After confirmation of sustained release of protein, osteogenic potential of the co-electrospun PRP/polymer scaffolds was evaluated by measuring relative gene expression, calcium content, and alkaline phosphatase (ALP) activity. Alizarin red and Hematoxylin and Eosin staining were performed as well. The results of ALP activity and calcium content demonstrated the effectiveness of PRP when c...Continue Reading

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Citations

Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Nov 18, 2019·Odontology·Shilpa TrivediSatyendra Kumar Singh
Nov 30, 2019·Stem Cells International·Gabriele StortiValerio Cervelli
May 10, 2020·Tissue Engineering. Part B, Reviews·Jie FangYu Wang

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