Enhanced osteoinduction of electrospun scaffolds with assemblies of hematite nanoparticles as a bioactive interface

International Journal of Nanomedicine
Shanshan MaYang Xia

Abstract

Electrospun scaffolds have been studied extensively for their potential use in bone tissue engineering. However, their hydrophobicity and relatively low matrix stiffness constrain their osteoinduction capacities. In the present study, we studied polymer electrospun scaffolds coated with hydrophilic hematite nanoparticles (αFeNPs) constructed using layer-by-layer (LbL) assembly to construct a bioactive interface between the scaffolds and cells, to improve the osteoinduction capacities of the scaffolds. The morphology of the αFeNPs was assessed. Surface properties of the scaffolds were tested by X-ray photoelectron spectroscopy (XPS), surface water contact angle, and in vitro protein adsorption test. The stiffness of the coating was tested using an atomic force microscope (AFM). In vitro cell assays were performed using rat adipose-derived stem cells (ADSCs). Morphology characterizations showed that αFeNPs assembled on the surface of the scaffold, where the nano assemblies improved hydrophilicity and increased surface roughness, with increased surface stiffness. Enhanced initial ADSC cell spread was found in the nano assembled groups. Significant enhancements in osteogenic differentiation, represented by enhanced alkaline phospha...Continue Reading

Citations

Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Peng WangJi-Hua Li

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Methods Mentioned

BETA
transmission electron microscopy
dynamic light scattering
scanning electron microscopy
AFM
X-ray
Protein Assay
enzyme-linked immunosorbent assay
Assay
infrared spectroscopy

Software Mentioned

Image
SPSS
CasaXPS
Pro Plus
WSxM
Casa

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